Marine fish protein peptide and preparation method thereof

Abstract

The invention discloses a marine fish protein peptide and a preparation method thereof. The steps of the method are: rinsing the fish meat with alkaline salt water and water successively, adding water to raise the temperature to 85-95°C, heating for 10-25min, and quickly cooling down to 2-2. Set aside at 5°C, discard the supernatant and grease to obtain the protein; adjust the protein concentration in the system to 30-50% w / v, then raise the temperature to 45-60°C, add trypsin 100-150U in sequence according to the weight of the protein / g, Bacillus subtilis protease 250-450U / g, control the concentration of solids to 10-15%, inactivate the enzyme, centrifuge the hydrolyzate to obtain the enzymatic solution; then filter through ceramic membrane, organic ultrafiltration membrane, nanofiltration membrane . This method breaks the traditional fish collagen preparation mode and technology, the equipment is simple, easy to operate, and suitable for large-scale production; the prepared protein peptide has good product activity, high quality, stable quality, and has good immune regulation and anti-fatigue , Antioxidant effect.

Description

technical field [0001] The invention relates to the field of marine biological products and biotechnology processing, in particular to a marine fish protein peptide and a preparation method thereof. Background technique [0002] Protein peptide (IGF-1), also known as human growth factor, is an active protein polypeptide substance, a molecular polymer between amino acids and proteins, mainly composed of 2 to 10 amino acids, it is the liver cells in the human body, The autocrine and paracrine products of more than a dozen kinds of cells such as kidney cells and spleen cells have very important biological significance. , promote growth, promote cell differentiation, wound repair, etc. In recent years, as the value of protein peptides has been paid more and more attention, extensive research has been carried out on the functional activities and preparation processes of protein peptides from different raw materials at home and abroad. The relatively mature protein peptide produc...

AI Technical Summary

Problems solved by technology

But at present, the proportion of deep processing of marine fish protein resources is low, and resources are seriously wasted

In the fish protein deep processing industry, fish collagen peptide is a relatively mature product at present, but the part of its development and utilization is the extracellular protein—collagen in the muscle matrix protein, and the intracellular peptide with high active ingredients Proteins—myofibrillar proteins and sarcoplasmic proteins have not been developed and utilized. The main reason is that there have always been technical bottlenecks in the removal of protein oil in cells, removal of fishy bitterness, and color. Therefore, the prepared marine fish protein peptide products The quality cannot meet the existing market requirements, which makes it difficult to industrialize

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