Method for detecting chloroplast DNAs to identify cytoplasmic male sterile line of hot pepper

Abstract

The invention discloses a method for detecting chloroplast DNAs to identify cytoplasmic male sterile line of hot pepper. The method comprises the following steps: taking the leaves of hot pepper as a material, extracting total DNAs in the leaves, carrying out PCR amplification by using chloroplast primers (C1 and C2), carrying out clone sequencing on amplification products, and then making a comparison with chloroplast DNA molecular fingerprints of the cytoplasmic male sterile line of hot pepper, namely identifying the cytoplasmic male sterile line of hot pepper simply, efficiently and accurately. The method for detecting chloroplast DNAs to identify the cytoplasmic male sterile line of hot pepper disclosed by the invention, the clone sequencing is carried out on the PCR amplification products of the chloroplast primers (C1 and C2) only when the cytoplasmic male sterile line of hot pepper is identified, and then a comparison is made, so that the cytoplasmic male sterile line of hot pepper can be identified simply, efficiently and accurately.

Description

technical field [0001] The invention relates to a method for detecting chloroplast DNA to identify cytoplasmic male sterile lines of peppers, belonging to the technical field of cytoplasmic male sterile detection. Background technique [0002] Cytoplasmic male sterility is a common phenomenon in the plant world. The use of male sterility for hybrid breeding has great advantages, such as no need for artificial emasculation, improving the purity of hybrid seeds and increasing yield; the mechanism of cytoplasmic male sterility is very complicated. No two cytoplasmic male sterility mutations were found to be identical. Molecular markers of cytoplasmic male sterile lines have been established in many plants, and the markers can be used to quickly distinguish sterile and fertile materials; studies on the genetic material of pepper male sterile lines are mainly concentrated in the nucleus, and rarely in the cytoplasm According to the report of Pepper Cytoplasmic Male Sterile Lines...

AI Technical Summary

Problems solved by technology

Molecular markers of cytoplasmic male sterile lines have been established in many plants, and the markers can be used to quickly distinguish sterile and fertile materials; studies on the genetic material of pepper male sterile lines are mainly concentrated in the nucleus, and rarely in the cytoplasm reported that there are fewer chloroplast DNA molecular markers that can be used to identify cytoplasmic male sterile lines of peppers, which increases the difficulty of breeding and identification of cytoplasmic male sterile lines of peppers and limits the development of hybrid breeding of peppers

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