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A method for increasing the yield of purple non-sulfur bacteria 5-aminolevulinic acid

A technology of aminolevulinic acid yield, purple non-sulfur bacteria, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of limited ability to degrade organic wastewater, 5-aminolevulinic acid yield Low, hinder the 5-aminolevulinic acid recycling process and other problems, to achieve the effect of enhancing the degradation ability, facilitating promotion, and improving energy metabolism activity

Active Publication Date: 2018-06-19
HARBIN INST OF TECH
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  • Summary
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  • Application Information

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Problems solved by technology

However, due to the limited ability of Rhodobacter sphaeroides to degrade organic wastewater, the yield of 5-aminolevulinic acid in the bacteria is low, which hinders the recovery of high-value 5-aminolevulinic acid in the treatment of food wastewater by Rhodobacter sphaeroides Acid Recycling Process

Method used

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  • A method for increasing the yield of purple non-sulfur bacteria 5-aminolevulinic acid
  • A method for increasing the yield of purple non-sulfur bacteria 5-aminolevulinic acid
  • A method for increasing the yield of purple non-sulfur bacteria 5-aminolevulinic acid

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specific Embodiment approach 1

[0021] Specific embodiment one: this embodiment improves purple non-sulfur bacterial cell 5-aminolevulinic acid productive rate according to the following steps:

[0022] 1. Adjust the pH value of the organic wastewater to 6.5-7.5, and then add metal ions Fe to the organic wastewater 2+ , and then add purple non-sulfur bacteria to make the initial fermentation solution;

[0023] 2. Ferment the initial fermentation liquid in step 1 under the conditions of light intensity of 1000-3000 lux, dissolved oxygen concentration controlled at 0.5-1.0 mg / L, and temperature of 25-30°C to realize purple non-sulfur bacterial cell 5- Increased yield of aminolevulinic acid;

[0024] Wherein, the metal ion Fe in the initial fermentation liquid of step 1 2+ The concentration is 50μmol / L~600μmol / L;

[0025] Step 1 The COD content of the organic wastewater is 6000-10000 mg / L, the TN content is 400-800 mg / L, and the TP content is 20-60 mg / L.

[0026] The purple non-sulfur bacteria (PNSB) used i...

specific Embodiment approach 2

[0027] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the metal ion Fe in the initial liquid of step 1 fermentation 2+ The concentration is 100μmol / L~500μmol / L. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0028] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the metal ion Fe in the initial liquid of step 1 fermentation 2+ The concentration is 200μmol / L~450μmol / L. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a method for increasing the yield of 5-aminolevulinic acid in purple non-sulfur bacteria, which relates to a method for increasing the yield of 5-aminolevulinic acid in organisms. It increases the yield of 5-aminolevulinic acid according to the following steps: 1. Adjust the pH value of the organic wastewater to 6.5-7.5, then add metal ions Fe2+ to the organic wastewater, and then add purple non-sulfur bacteria to make the initial fermentation solution 2. Fermenting the initial fermentation liquid in step 1 under the conditions that the light intensity is 1000-3000 lux, the dissolved oxygen concentration is controlled at 0.5-1.0 mg / L, and the temperature is 25-30°C. Compared with the existing method for biosynthesizing 5-aminolevulinic acid, the method of the present invention has significantly improved bacterial cell yield and 5-aminolevulinic acid yield, and the COD removal rate of wastewater also reaches more than 90%.

Description

technical field [0001] The invention relates to a method for increasing the yield of 5-aminolevulinic acid in organisms. Background technique [0002] 5-Aminolevulinic acid (ALA) is the biological synthesis of chlorophyll, heme, porphyrin and vitamin B 12 Precursors of tetrapyrrole compounds, such as tetrapyrrole compounds, widely exist in microorganisms, plants and animal cells. In 1999, a photodynamic drug with ALA as the main component was approved by the Food and Drug Administration (USA) for the treatment of actinic keratosis. In addition, ALA has broad prospects for clinical application in the field of photodynamic diagnosis and treatment of diseases such as cancer and oral verrucous hyperplasia. At the same time, as a new type of photoactivated pesticide with high environmental compatibility and selectivity, ALA is harmless to crops, humans and animals, easy to degrade, and non-polluting, and can be widely used as photodynamic insecticides and herbicides in agricult...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00C12R1/01
Inventor 刘淑丽张光明李相昆张杰
Owner HARBIN INST OF TECH
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