Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for improving yield of purple non-sulfur bacteria thallus 5-aminolevulinic acid

A technology of aminolevulinic acid yield and purple non-sulfur bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low yield of 5-aminolevulinic acid and the ability to degrade organic wastewater limited, hindering the process of resource utilization of 5-aminolevulinic acid, etc., to achieve the effects of easy promotion, increased yield, and simple and easy method

Active Publication Date: 2015-04-22
HARBIN INST OF TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limited ability of Rhodobacter sphaeroides to degrade organic wastewater, the yield of 5-aminolevulinic acid in the bacteria is low, which hinders the recovery of high-value 5-aminolevulinic acid in the treatment of food wastewater by Rhodobacter sphaeroides Acid Recycling Process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving yield of purple non-sulfur bacteria thallus 5-aminolevulinic acid
  • Method for improving yield of purple non-sulfur bacteria thallus 5-aminolevulinic acid
  • Method for improving yield of purple non-sulfur bacteria thallus 5-aminolevulinic acid

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0021] Specific embodiment one: this embodiment improves purple non-sulfur bacterial cell 5-aminolevulinic acid productive rate according to the following steps:

[0022] 1. Adjust the pH value of the organic wastewater to 6.5-7.5, and then add metal ions Fe to the organic wastewater 2+ , and then add purple non-sulfur bacteria to make the initial fermentation solution;

[0023] 2. Ferment the initial fermentation liquid in step 1 under the conditions of light intensity of 1000-3000 lux, dissolved oxygen concentration controlled at 0.5-1.0 mg / L, and temperature of 25-30°C to realize purple non-sulfur bacterial cell 5- Increased yield of aminolevulinic acid;

[0024] Wherein, the metal ion Fe in the initial fermentation liquid of step 1 2+ The concentration is 50μmol / L~600μmol / L;

[0025] Step 1 The COD content of the organic wastewater is 6000-10000 mg / L, the TN content is 400-800 mg / L, and the TP content is 20-60 mg / L.

[0026] The purple non-sulfur bacteria (PNSB) used i...

specific Embodiment approach 2

[0027] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the metal ion Fe in the initial liquid of step 1 fermentation 2+ The concentration is 100μmol / L~500μmol / L. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0028] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the metal ion Fe in the initial liquid of step 1 fermentation 2+ The concentration is 200μmol / L~450μmol / L. Other steps and parameters are the same as those in Embodiment 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for improving yield of purple non-sulfur bacteria thallus 5-aminolevulinic acid, and relates to a method for improving yield of organism 5-aminolevulinic acid. The method is used for improving the yield of 5-aminolevulinic acid according to the following steps: 1, regulating the pH value of organic wastewater to 6.5-7.5, throwing metal ions Fe<2+> into the organic wastewater, and throwing purple non-sulfur bacteria to prepare primary fermentation broth; and 2, fermenting the primary fermentation broth obtained in the step 1 at light intensity of 1000-3000 lux, a dissolved oxygen concentration of 0.5-1.0 mg / L and a temperature of 25-30 DEG C. Compared with the existing method for biologically synthesizing 5-aminolevulinic acid, by adopting the method provided by the invention, the yield of thallus and the yield of 5-aminolevulinic acid are obviously improved, and the removal rate of wastewater COD is higher than 90%.

Description

technical field [0001] The invention relates to a method for increasing the yield of 5-aminolevulinic acid in organisms. Background technique [0002] 5-Aminolevulinic acid (ALA) is the biological synthesis of chlorophyll, heme, porphyrin and vitamin B 12 Precursors of tetrapyrrole compounds, such as tetrapyrrole compounds, widely exist in microorganisms, plants and animal cells. In 1999, a photodynamic drug with ALA as the main component was approved by the Food and Drug Administration (USA) for the treatment of actinic keratosis. In addition, ALA has broad prospects for clinical application in the field of photodynamic diagnosis and treatment of diseases such as cancer and oral verrucous hyperplasia. At the same time, as a new type of photoactivated pesticide with high environmental compatibility and selectivity, ALA is harmless to crops, humans and animals, easy to degrade, and non-polluting, and can be widely used as photodynamic insecticides and herbicides in agricult...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12R1/01
Inventor 刘淑丽张光明李相昆张杰
Owner HARBIN INST OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com