Method for culturing and detecting lung cancer cells based on microfluidic chip

Abstract

The invention discloses a method for cultivating and detecting lung cancer cells based on a micro-fluidic chip. The method comprises the following steps: 1) disinfecting the micro-fluidic chip: disinfecting the micro-fluidic chip in ethanol, radiating the micro-fluidic chip under ultraviolet light for 1h and rinsing the micro-fluidic chip in phosphate buffer saline (PBS); 2) inoculating and cultivating lung cancer cells: preparing a cell suspension from the cultivated lung cancer cells and vertically injecting the cell suspension from an inlet; cultivating in an incubator for 30min, taking out the suspension, injecting a fresh culture medium and cultivating in the incubator once again; and 3) detecting a lung cancer cell metabolite: observing the morphology and growing status of the lung cancer cells every other 8hr, taking pictures, and detecting the lung cancer cells in a metabolic fluid collecting pool by virtue of a prepared porphyrin sensing chip. The method disclosed by the invention can be used for detecting the physiological difference of the lung cancer cells in real time and can be used for distinguishing the different metabolic fluids of the different types of lung cancer cells; and the cellular metabolic fluid cultivated by virtue of the micro-fluidic chip disclosed by the invention keeps a higher similarity with the metabolic liquid generated from a human tissue, and the cellular metabolic fluid is more suitable for researching a marker for the early diagnosis of lung cancer.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a method for culturing and detecting lung cancer cells based on microfluidic chips. Background technique [0002] Lung cancer, also known as bronchial cancer, occurs in the epithelium of the bronchial mucosa. It is one of the malignant tumors with the fastest-growing morbidity and mortality and the greatest threat to the health and life of the population. Because the symptoms of lung cancer are not obvious in the early stage, it is often discovered at an advanced stage. Therefore, early diagnosis and early treatment of lung cancer are the most effective ways to prevent and treat lung cancer and reduce the mortality rate. At present, clinically, the common diagnostic methods for lung cancer are as follows: X-ray detection, computerized tomography (CT), magnetic resonance tomography (MRI), sputum exfoliated cell examination, fiber bronchial examination, percutaneous lung...

AI Technical Summary

Problems solved by technology

However, microfluidic chip technology is still in its infancy, and its related technologies and methods for lung cancer cell culture and detection have not been reported yet.

Images