Culture medium for culturing virus and preparation method of culture medium

A culture medium and virus technology, applied in the field of virus culture medium and its preparation, can solve the problems of many steps, increased production costs, complicated operation methods, etc., and achieve the effect of simplifying the process, reducing difficulty, and realizing large-scale production

Active Publication Date: 2015-04-29
ZHAOQING DAHUANONG BIOLOGIC PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can remove impurities very well, the operation method is complicated and has many steps, which increases the production cost

Method used

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  • Culture medium for culturing virus and preparation method of culture medium
  • Culture medium for culturing virus and preparation method of culture medium
  • Culture medium for culturing virus and preparation method of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of culture medium that is used for cultivating virus, comprises DMEM medium (culture fluid), and it also comprises: the small molecule component in amino acid solution and fetal calf serum; The small molecule component in described DMEM medium, amino acid solution and fetal calf serum The molecular components are calculated by volume percentage: 93% of DMEM medium, 2% of amino acid solution, and 5% of small molecular components in fetal bovine serum; the molecular weight of the small molecular components in fetal bovine serum is ≤30KDa;

[0022] The solvent of the amino acid solution is DMEM medium, and the solute is composed of the following components in terms of mass percentage: 0.3% glycine, 0.7% isoleucine, 0.05% serine, 0.1% alanine, and 0.2% phenylalanine , Tyrosine 0.2%, Valine 0.4%, Proline 0.03%, Cysteine ​​0.2%, Leucine 0.4%, Tryptophan 0.5%, Methionine 0.7%, Asparagine 0.06%, Glutamine Amide 1%, Threonine 0.3%, Aspartic Acid 0.08%, Glutamic Acid 0.5%,...

Embodiment 2

[0030] A kind of culture medium that is used for cultivating virus, comprises MEM medium (culture fluid), and it also comprises: the small molecule component in amino acid solution and fetal calf serum; The small molecule component in described MEM medium, amino acid solution and fetal calf serum The molecular components are as follows in terms of volume percentage: 88% of MEM medium, 2% of amino acid solution, and 10% of small molecular components in fetal bovine serum; the molecular weight of the small molecular components in fetal bovine serum is ≤30KDa;

[0031]The solution of the amino acid solution is a MEM medium solution, and the solute is composed of the following components in terms of mass percentage: 0.1% glycine, 0.5% isoleucine, 0.1% serine, 0.2% alanine, 0.1% phenylalanine %, tyrosine 0.2%, valine 0.3%, proline 0.02%, cysteine ​​0.2%, leucine 0.2%, tryptophan 0.6%, methionine 0.6%, asparagine 0.06%, gluten Aminoamide 0.7%, Threonine 0.3%, Aspartic Acid 0.06%, Gl...

Embodiment 3

[0039] A kind of culture medium that is used for cultivating virus, comprises DMEM medium (culture fluid), and it also comprises: the small molecule component in amino acid solution and fetal calf serum; The small molecule component in described DMEM medium, amino acid solution and fetal calf serum The molecular components are as follows in terms of volume percentage: 85% of DMEM medium, 5% of amino acid solution, and 10% of small molecular components in fetal bovine serum; the molecular weight of the small molecular components in fetal bovine serum is ≤30KDa;

[0040] The solvent of the amino acid solution is DMEM medium, and the solute is composed of the following components in terms of mass percentage: 1% glycine, 1.5% isoleucine, 1% serine, 1% alanine, and 0.3% phenylalanine , Tyrosine 2%, Valine 1%, Proline 0.5%, Cysteine ​​1%, Leucine 1%, Tryptophan 2%, Methionine 1%, Asparagine 0.3%, Glutamine Amide 2%, Threonine 1.2%, Aspartic Acid 0.2%, Glutamic Acid 0.2%, Lysine 1.5%...

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Abstract

The invention relates to a culture medium for culturing a virus. The culture medium comprises the following components respectively in percent by volume: 85-96 percent of a culture solution, 2-5 percent of an amino acid solution and 2-10 percent of small molecular components in fetal calf serum. The invention further provides a preparation method of the culture medium. The preparation method comprises the following steps: mixing the culture solution, the amino acid solution and the small molecular components in the fetal calf serum according to formula amounts to obtain the culture medium. The culture medium for culturing the virus, disclosed by the invention, facilitates the purification of a virus solution subjected to virus proliferation, and the production cost of vaccines is reduced; in addition, the preparation method is simple and easy to operate.

Description

technical field [0001] The invention relates to a medium and a preparation method thereof, in particular to a medium for culturing viruses and a preparation method thereof. Background technique [0002] Since 1996, mammalian cell culture has been developed to allow free scale-up and industrial production. Many biologically active substances, vaccines, and carriers, such as monoclonal antibodies, pharmaceutical proteins, and PRRS vaccines, can all be obtained through large-scale culture of animal cells. The production of biological products through cell culture can not only improve the quality of biological products, but also promote the development of cell culture technology, protein expression and purification technology, and virus culture technology. Judging from the current development trend, cell suspension culture and serum-free culture are the development direction of industrial production of major biological companies around the world in recent years. [0003] At pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12R1/93
Inventor 陈瑞爱徐家华蒋春英王新秋张东霞
Owner ZHAOQING DAHUANONG BIOLOGIC PHARMA
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