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Cultivating method of wheat-agropyron elongatum FHB (Fusarium head blight)-resistant 7E chromosome long arm translocation line

A technology of Thinopyrum elongatum and scab, which is applied to the field of wheat-Thnature elongatum resistance to head blight 7E chromosome long-arm translocation line, can solve the problem of diploid Thinpyrum elongatum, which is rarely seen. Departmental research reports and other issues

Inactive Publication Date: 2015-05-06
YANGZHOU UNIV
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Problems solved by technology

[0013] 4.1.1 Spontaneous translocation
However, most of the researches on the genus of Thipotinium are on Triniprum intermedium and Decaploid Triniprum elongatum, but there are few reports on translocation lines of diploid Triniprum elongatum.

Method used

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  • Cultivating method of wheat-agropyron elongatum FHB (Fusarium head blight)-resistant 7E chromosome long arm translocation line
  • Cultivating method of wheat-agropyron elongatum FHB (Fusarium head blight)-resistant 7E chromosome long arm translocation line
  • Cultivating method of wheat-agropyron elongatum FHB (Fusarium head blight)-resistant 7E chromosome long arm translocation line

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Embodiment 1

[0054] 1. Experimental materials

[0055] Common wheat China Spring (CS), diploid Enopyrum elongatum (EE) (2X), China Spring-Etonia elongatum disomic substitution lines: DS7E7A, DS7E7B; : DA7E, DA7EL, DA7ES (the above 5 materials were donated by Dr.Fedax of the Canadian Department of Agriculture, and the original document of the materials is "Disomic and ditelosomic additions ofdiploidAgropyron elongatum chromosomes to Triticum aestivum"); Yangmai 16 (Y16, namely Yang 0-126 It is a hybrid of Yang 91F138 and Yang 90-30 used by the Lixiahe Agricultural Science Institute of Jiangsu Province), and the above materials are provided by the Lixiahe Agricultural Science Institute of Jiangsu Province, all of which are commercially available; Annong 8455 (An8455, bred by Anhui Academy of Agricultural Sciences) , Sumai 3 (Su3, Suzhou Institute of Agricultural Sciences bred in 1968 using Funo and Taiwan wheat) number is the laboratory's own varieties).

[0056] 2. Genomic DNA extraction ...

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Abstract

The invention relates to a cultivating method of a wheat-agropyron elongatum FHB (Fusarium head blight)-resistant 7E chromosome long arm translocation line. The cultivating method comprises the following steps: processing F2-generation seeds obtained by hybridizing a wheat-agropyron elongatum 7E substitution line and Yangmai 16 by <60>Co radiation, and in a propagation process of radiated offspring, firstly, performing field FHB resistance screening to select individual plants which are high in FHB resistance and have related features of agropyron elongatum; and based on this, detecting the obtained individual plants by using an agropyron elongatum 7E chromosome long arm specific marker, and finally, confirming the wheat-agropyron elongatum FHB-resistant translocation line by virtue of cytology. By combining field phenotype screening, molecular marker identification and cytology confirmation, the wheat-agropyron elongatum FHB-resistant 7E chromosome long arm translocation line is created; the obtained FHB-resistant translocation line provides a new germplasm or base material for wheat variety improvement and genetic research, and can be used as a parent to participate in cultivation of the FHB-resistant wheat.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and this research utilizes 60 Co (cobalt 60) radiation treated the F2 generation seeds of wheat-Ethiopyra elongatum 7E substitution line (DS7E / 7B) crossed with Yangmai 16. During the reproduction process of the irradiated offspring, the resistance to head blight in the field was screened first. Individual plants with better resistance to scab and E. elongatum characteristics were selected for planting. On this basis, the selected individual plants were detected by using the specific marker on the long arm of chromosome 7E of E. elongatum. Finally, wheat- Confirmation of translocation line resistant to head blight of E. elongatum. Using the combination of field phenotype screening, molecular marker identification and cytological confirmation, a wheat-Thinopyrum elongatum resistance to head blight 7E chromosome long arm translocation line was established. Background technique [0002] 1 ...

Claims

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Application Information

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IPC IPC(8): A01H1/02C12Q1/68
Inventor 陈建民张璐璐高勇陈士强刘慧萍黄泽峰秦树文黄帅
Owner YANGZHOU UNIV
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