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A high-yield oil-producing Scenedesmus and its screening, cultivation methods and applications

A high-yield technology for Scenedesmus, applied in the field of culture, high-yield Scenedesmus and its screening, can solve the problems of high cost of microalgae cultivation, restriction of commercial production of microalgae oil production, low oil content of microalgae, etc. The effect of accumulating properties

Active Publication Date: 2019-01-29
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low oil content of some microalgae and the high cost of microalgae culture, these two problems restrict the large-scale commercial production of oil produced by microalgae.

Method used

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  • A high-yield oil-producing Scenedesmus and its screening, cultivation methods and applications
  • A high-yield oil-producing Scenedesmus and its screening, cultivation methods and applications
  • A high-yield oil-producing Scenedesmus and its screening, cultivation methods and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The isolation and identification of embodiment 1 Scenedesmus SDEC-12:

[0029] 1. Sample collection

[0030] Water samples were collected from Yingxue Lake (polluted lake) of Shandong Jianzhu University on December 11, 2012.

[0031] 2. Isolation and purification of Scenedesmus SDEC-12

[0032] Separation and purification steps: the retrieved water sample was centrifuged and concentrated, then added to BG-11 medium for expanded culture, counted under a microscope by hemocytometer after about 7 days, and then diluted the density of the algae liquid to about 4000 / mL according to the counted amount . On the ultra-clean test bench, pour the prepared semi-solid medium (BG-11 culture solution + 0.5-0.6% agar) onto the plate (it has been placed overnight, no bacteria grow), and the diluted algae liquid is used for medical Throat sprayers were sprayed on the marked plates, then covered, and placed in a light incubator for more than ten days, and microscopic examination was p...

Embodiment 2

[0043] Embodiment 2 Growth characteristics of Scenedesmus SDEC-12 in artificially configured domestic sewage

[0044] The growth characteristics of Scenedesmus SDEC-12 in BG-11 medium and artificial sewage were compared.

[0045] (1) The medium used is BG-11 and artificially prepared domestic sewage

[0046] The components of BG-11 medium and domestic sewage prepared artificially were as described above, and both mediums were sterilized by autoclaving.

[0047] (2) Growth of Scenedesmus SDEC-12 in two media

[0048] Scenedesmus SDEC-12 was expanded to an initial inoculation density of 10 6 Each / mL were respectively inoculated in BG-11 medium (control group) and artificially prepared domestic sewage (experimental group), and then cultured in a light incubator at a temperature of 25±1°C under full light and a light intensity of 4000 lux. . Daily monitoring of water quality indicators including ammonia nitrogen, total nitrogen, total phosphorus and pH value. During the cultu...

Embodiment 3

[0050] Example 3 Oil production characteristics of Scenedesmus SDEC-12

[0051] 1. Acquisition of Scenedesmus SDEC-12 algae powder

[0052] Cultivate Scenedesmus SDEC-12 according to the conditions and medium in Example 2, and harvest the algae by centrifugation when the algae grows to the stationary phase, and process the harvested algae mud in a freeze dryer to obtain dry algae powder.

[0053] 2. Determination of growth and oil production of Scenedesmus SDEC-12

[0054] 1. Determination of Scenedesmus SDEC-12 biomass

[0055] By monitoring the biomass concentration of algae cells every day, the algae liquid is harvested by centrifugation when the growth stage of algae cells enters a stable period. Record the volume of the harvested algae liquid, use a 50mL centrifuge tube to hold the algae liquid, put it in a large-capacity refrigerated centrifuge for centrifugation, remove the supernatant and retain the sediment, and dry at 60°C to obtain algal powder. The biomass is ca...

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Abstract

The invention discloses a highly oil-yielding Scenedesmus strain, its screening, its cultivation method and its application. According to the 18S rDNA sequence analysis and microscopic identification of the algae isolated from the polluted waters, the algae was identified as a member of the genus Scenedesmus sp., and its sequence number was KJ413176. The algae strain is named Scenedesmus bijuga SDEC‑12 (Scenedesmus bijuga SDEC‑12), and it has been preserved in China Center for Type Culture Collection in October 2014, and its preservation number is CCTCC NO: M 2014497. Using artificially prepared domestic sewage as an alternative medium, the oil production and oil yield of Scenedesmus SDEC‑12 were significantly higher than that of the more commonly used BG‑11 medium, and the analysis of the extracted fatty acid methyl ester components showed that after living Scenedesmus SDEC‑12 cultivated in sewage has better oxidation stability and low temperature fluidity, so it is more suitable as a raw material for biodiesel. Scenedesmus SDEC‑12 grows while absorbing nitrogen and phosphorus nutrients in domestic sewage to achieve effective treatment of domestic sewage. Therefore, using domestic sewage as an alternative medium has both economic and environmental benefits.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to a high-yield oil-yielding Scenedesmus and its screening, cultivating method and application. Background technique [0002] With the rapid development of economy and society, human consumption and demand for fossil fuels such as coal, oil, and natural gas are increasing. However, such fossil fuels are non-renewable. According to the survey results of proven oil reserves in various countries in the world in 2009, the total amount of oil in the world is 1342.2×10 9 barrels, which can be used for 40 years, but the total amount of oil in China is only 16×10 9 barrels, ranking 13th in the world, so it is imminent to find renewable alternative new energy sources. In recent years, microalgae oil production has become a research hotspot in various countries. As a raw material for biodiesel production, microalgae has the advantages of strong environmental adaptability,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C02F3/32B01D53/84C12P7/64C12R1/89
CPCB01D53/84C02F3/322C12N1/12C12P7/6409C12P7/649C12N1/125C12R2001/89Y02E50/10Y02A50/20
Inventor 裴海燕韩琳张硕宋明明韩飞
Owner SHANDONG UNIV
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