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Method for detecting triglyceride in serum

A technology of triglyceride and triglyceride, which is applied in the field of bioanalytical chemistry and can solve undisclosed and other problems

Active Publication Date: 2015-05-06
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The prior art does not disclose a technical solution for detecting the activity of lipase by using dopamine as the carrier for immobilizing lipase and reducing chloroauric acid in situ to form gold nanoparticles as signal enhancing elements

Method used

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  • Method for detecting triglyceride in serum
  • Method for detecting triglyceride in serum
  • Method for detecting triglyceride in serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Preparation of polydopamine-nanogold / ITO electrode

[0039] Immerse the pretreated ITO electrode into a dopamine solution with a volume of 1 mL and a concentration of 2 mg / mL, add 0.1 mol / L Tris-HCl buffer solution to it, adjust the pH of the solution to 8.5, and use a pipette gun to Add 0.1mol / L chloroauric acid solution to the solution to make the final concentration 5μmol / L, mix well, and react at room temperature for 4 hours, take out the ITO electrode with tweezers, wash it with double distilled water three times, and you can get polydopamine-nano Gold-modified ITO electrodes.

[0040] (2) Lipase modification onto the surface of polydopamine-nanogold / ITO electrode

[0041] Add 20 μL, 2 mg / mL lipase solution dropwise to the surface of the polydopamine-nanogold / ITO electrode obtained in step (1), spread it evenly with a pipette tip, and then place it in a refrigerator (4° C.) to dry naturally in the shade for 24 hours. Wash three times with phosphate buffer sol...

Embodiment 2

[0043] Electrochemical detection of triglycerides

[0044] The bioenzyme electrode modified in Example 1 was placed in a solution containing a certain concentration of triglyceride, and reacted at 37°C for 25 minutes. After the reaction, the electrode was cleaned with ultrapure water, and potassium ferricyanide solution was added to the reaction solution to make Its final concentration is 5mmol / L, carries out electrochemical cyclic voltammetry scanning then, obtains the CV peak current corresponding to different concentrations of triglycerides (see Figure 4 ), and then take the reduction peak current as the ordinate, and the concentration of triglyceride as the abscissa, draw the standard curve of triglycerides (see Figure 5 ).

Embodiment 3

[0046] Determination of triglyceride content in serum

[0047] Determination of triglyceride content in serum, what we adopted is the standard addition method, the serum of the healthy person that has been processed is diluted 50 times, then adds the triglyceride (50,100,150,200mg / mL) of different concentrations wherein, The corresponding current signal value is measured, and compared with the standard curve obtained in Example 2, the recovery rate of triglyceride can be obtained.

[0048] Triglyceride concentration (mg.dL -1 )

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Abstract

The invention belongs to the field of bio-analytical chemistry, and relates to an electrochemical method for detecting triglyceride in serum. According to the present invention, the characteristic that dopamine is automatically polymerized under a weakly alkaline condition to form polydopamine is utilized, wherein the formed polydopamine is used for immobilizing enzyme molecules, chloroauric acid is subjected to in situ reduction to produce nanometer gold so as to be adopted as the signal amplifying element, and the cyclic voltammetry (CV) is adopted as the electrochemical detection method; and the developed electrochemical sensing strategy for detecting the triglyceride in the serum has advantages of high sensitivity, simple operation and the like, and is suitable for promotion and application.

Description

technical field [0001] The invention belongs to the field of bioanalytical chemistry, relates to a method for detecting triglyceride in serum, and specifically relates to using polydopamine to efficiently immobilize biological enzyme molecules on the one hand, and on the other hand to reduce chloroauric acid in situ during the polymerization process of dopamine. An electrochemical sensing strategy with signal amplification for highly sensitive detection of triglycerides in serum. Background technique [0002] Triglyceride (Triglyceride, TG), also known as neutral fat, is formed by the esterification of three molecules of fatty acid and one molecule of glycerol, and is the main source of energy in the body. The level of triglycerides is not only positively correlated with total cholesterol (TC), body mass index (BMI) and blood glucose (GLU), but also closely related to obesity. The detection of triglycerides in human blood is very important because of its high concentration ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
Inventor 田丹碧张卫黄和江凌汤燕刘佳甘建红
Owner NANJING TECH UNIV
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