A biochip for enrichment of tumor markers and its application
A technology of tumor markers and biochips, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of decreased concentration of tumor markers, affecting the sensitivity of detection methods, and limiting the application requirements of tumor detection, so as to promote high-efficiency enrichment, Meet the needs of subsequent detection applications and avoid losses
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Embodiment 1
[0049]A biochip for enriching tumor markers, comprising a substrate 9 and a microfluidic system 10, the microfluidic system 10 is distributed on the surface of the substrate 9, and the microfluidic system 10 sequentially includes an injection port 1, a channel 2, The reaction pool 3, the separation magnetic area 4, the collection pool 5, the fixed magnetic area 6 and the sample outlet 7, and the sample inlet 1 communicate with the reaction pool 3 through the passage 2, and the passage 2 is divided into two branches after passing through the reaction pool 3, Wherein the first branch is communicated with the collection pool 5, the second branch is communicated with the sample outlet 7, the separation magnetic region 4 is located on one side of the first branch, and the fixed magnetic region 6 surrounds the collection pool 5; the reaction pool 3 is distributed with Antibodies labeled with magnetic nanoparticles are used as probes, and the magnetic nanoparticles and antibodies are ...
Embodiment 2
[0059] Example 2 Enrichment of alpha-fetoprotein (AFP):
[0060] (1) Preparation of biochip:
[0061] Preparation of the substrate structure: First, use acetone, isopropanol, and ultrapure water to ultrasonically clean the silicon wafer for 15 minutes each, and then use a coating table to coat a layer of AZ5214 photoresist on the surface with a coating thickness of 1.6 μm. like image 3 As shown; the photoresist pattern is prepared on the surface of the silicon wafer by the ultraviolet lithography process, and the separation magnetic region and the fixed magnetic region are defined, such as Figure 4 Shown; further use of magnetron sputtering equipment to deposit magnetic Fe 3 o 4 Thin film with a deposition thickness of 0.5 μm; then use acetone and isopropanol to remove the AZ5214 glue by stripping process. Through the implementation of this process, separated magnetic regions and fixed magnetic regions can be prepared on the surface of silicon wafers, such as Figure ...
Embodiment 3
[0067] Example 3 Co-enrichment of carcinoembryonic antigen (CEA), heterogeneous alpha-fetoprotein (AFP-L3) and interleukin-6 (IL-6):
[0068] (1) Preparation of biochip:
[0069] Preparation of the substrate structure: First, use acetone, isopropanol, and ultrapure water to ultrasonically clean the silicon wafer for 15 minutes each, and use a coating table to coat a layer of AZ5214 photoresist on the surface with a coating thickness of 2 μm, such as image 3 As shown; the photoresist pattern is prepared on the surface of the silicon wafer by the ultraviolet lithography process, and the separation magnetic region and the fixed magnetic region are defined, such as Figure 4 As shown; further use magnetron sputtering equipment to deposit a magnetic FeCo thin film with a deposition thickness of 1 μm; and then use acetone and isopropanol to remove the AZ5214 glue by a stripping process. Through the implementation of this process, separated magnetic regions and fixed magnetic reg...
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