A Super-resolution Fluorescence Spectral Imaging Microscope

Abstract

The invention discloses a super-resolution fluorescence spectrum imaging microscope. The super-resolution fluorescence spectrum imaging microscope comprises a laser emitter, a dichroic mirror, a spectroscope, an image controller and a spectrograph, the laser emitter stimulates fluorophores of a sample to emit fluorescence, the spectroscope divides the fluorescence into a first beam of light path and a second beam of light path, both the first beam of light path and second beam of light path have fluorescence signals of the fluorescence and spurious signals of exciting light, the first beam of light path is emitted to the image controller to gather the intensity information of the fluorescence, the second beam of light path is emitted to the spectrograph to analyze the spectrum information of the fluorescence and distinguish fluorescence signals from different fluorophores in the fluorescence according to the spectrum information, and the spectrum information gathered by the spectrograph is used for distinguishing the fluorescence signals of the fluorescence and spurious signals of the exciting light so as to distinguish the exciting light from the fluorescence signals through the spectrograph and improve the imaging effect of the super-resolution fluorescence spectrum imaging microscope.

Description

Technical field [0001] The invention belongs to the field of image display technology, and in particular relates to a super-resolution fluorescence spectrum imaging microscope. Background technique [0002] In the field of cell biology, super-resolution fluorescence microscopy has important applications in the study of cell structure, function and various laws of life. However, due to the limitation of the optical system, the traditional super-resolution fluorescence microscope has a diffraction limit, which makes it difficult for its limit resolution to exceed 200nm. The super-resolution fluorescence microscopy technology can break through the limitation of diffraction limit in traditional optical systems and further improve the resolution. The use of super-resolution fluorescence microscopy allows scientists to study living cells at the molecular level, such as observing the microstructures of biological macromolecules and organelles in living cells and how cell functions are ...

AI Technical Summary

Problems solved by technology

This makes it impossible for the CCD to distinguish between the excitation light signal and the fluorescence signal when collecting the fluorescence signal, which will affect the sample image, resulting in a low signal-to-noise ratio for microscopic imaging

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