A Super-resolution Fluorescence Spectral Imaging Microscope
A super-resolution fluorescence and spectral imaging technology, applied in the field of image display, can solve the problems of inability to distinguish excitation light signal and fluorescence signal, and low signal-to-noise ratio of microscopic imaging, so as to improve the imaging effect and reduce the signal-to-noise ratio.
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[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention.
[0021] See figure 1 , The present invention provides a super-resolution fluorescence spectrum imaging microscope 100. The super-resolution fluorescence spectral imaging microscope 100 includes a laser transmitter 10, a dichroic mirror 20, a spectroscope 30, an image controller 40 and a spectrometer 50. The laser transmitter 10 emits excitation light to the dichroic mirror 20, and the dichroic mirror 20 reflects the excitation light to the sample 1 to excite the fluorophore of the sample 1 to emit fluorescence A, and the fluorescence A has fluorescence. Signals and stray signals of excitation light, the dichroic mirror 20 transmits the fluorescence A of the sample 1 to the spectroscope 30. The spectroscope 30 divides the fluorescent light A into a first light path A1 and a second ...
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