A kind of preparation method of α-glucosidase inhibitor and its product and application

A glucosidase and inhibitor technology, applied in the field of preparation of α-glucosidase inhibitors, can solve problems such as retention, lack of theoretical basis, and obstacles to the large-scale preparation and application of α-glucosidase inhibitors, and achieve control Effects of blood sugar fluctuations

Active Publication Date: 2017-07-21
BRIGHT DAIRY & FOOD CO LTD
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These studies all stay at the level of the crude extract of soybean fermented product, and there are few reports on the separation and characterization of the effective components of the inhibitor. Therefore, the lack of theoretical basis has greatly hindered the development of α-glucosidase inhibitors in soybean products. Large-scale preparation and application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of α-glucosidase inhibitor and its product and application
  • A kind of preparation method of α-glucosidase inhibitor and its product and application
  • A kind of preparation method of α-glucosidase inhibitor and its product and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The preparation of embodiment 1 two kinds of α-glucosidase inhibitors

[0053] Use Lactobacillus plantarum ST-Ⅲ with the preservation number CGMCC No.0847 as the fermentation strain to ferment 1L of aseptic soybean milk at 25°C for 24 hours. The obtained fermentation liquid is heated at 100°C for 10 minutes, cooled to 25°C and centrifuged to obtain the supernatant , centrifuged at 8000rpm for 10 minutes to get the supernatant obtained by extraction with 2 times the volume of ethyl acetate, and after the obtained ethyl acetate phase was rotatably evaporated to dryness, 20mL of ethyl acetate was added to prepare the crude extract of α-glucosidase inhibitors. solution, take 1.5mL of the crude extract and put it on the silica gel column whose initial condition is ethyl acetate: n-hexane volume ratio of 2:3 (v / v), according to the volume ratio of ethyl acetate: n-hexane : 3 (v / v), the volume ratio of ethyl acetate:n-hexane is 3:2 (v / v), the volume ratio of ethyl acetate:n-he...

Embodiment 2

[0055] The preparation of embodiment 2 two kinds of α-glucosidase inhibitors

[0056] Use Lactobacillus plantarum ST-Ⅲ with the preservation number CGMCC No.0847 as the fermentation strain to ferment 1L of aseptic soybean milk at 40°C for 120 hours. The obtained fermentation liquid is heated at 98°C for 20 minutes, cooled to 20°C and then centrifuged at 9000rpm for 15 minutes. Get supernatant, the supernatant of gained uses the ethyl acetate of 4 times volume to extract, after the ethyl acetate phase of gained is evaporated to dryness, add 20mL ethyl acetate and be mixed with the crude extract of α-glucosidase inhibitor, take this 1.5 mL of the crude extract was loaded on a silica gel column with an initial condition of ethyl acetate: n-hexane volume ratio of 2:3 (v / v), according to ethyl acetate: n-hexane volume ratio of 2:3 (v / v), ethyl acetate: the volume ratio of n-hexane is 3:2 (v / v), ethyl acetate: the volume ratio of n-hexane is 4:1 (v / v) order, with the flow rate of 1...

Embodiment 3

[0058] The preparation of embodiment 3 two kinds of α-glucosidase inhibitors

[0059] Use Lactobacillus plantarum ST-Ⅲ with preservation number CGMCC No.0847 as the fermentation strain to ferment 1L of aseptic soybean milk at 37°C for 72 hours, heat the obtained fermentation liquid at 95°C for 30min, cool to 15°C and centrifuge at 8000rpm for 10 minutes Get supernatant, the supernatant of gained uses the ethyl acetate of 5 times of volumes to extract, after the ethyl acetate phase of gained is evaporated to dryness, add 20mL ethyl acetate and be mixed with the crude extract of α-glucosidase inhibitor, take this 1.5 mL of the crude extract was loaded on a silica gel column with an initial condition of ethyl acetate: n-hexane volume ratio of 2:3 (v / v), according to ethyl acetate: n-hexane volume ratio of 2:3 (v / v), the volume ratio of ethyl acetate: n-hexane is 3:2 (v / v), the volume ratio of ethyl acetate: n-hexane is 4:1 (v / v) sequence, with a flow rate of 1.5mL / min Carry out...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of an α-glucosidase inhibitor and its product and application. The preparation method comprises the following steps: centrifuging fermented soybean milk to obtain the supernatant, extracting the obtained supernatant with ethyl acetate, and After the obtained ethyl acetate phase was evaporated to dryness under reduced pressure, ethyl acetate was added to be mixed with the crude extract of α-glucosidase inhibitor, and the gained crude extract was separated and eluted using a silica gel column, and the test gained eluent was effective against The inhibitory effect of α-glucosidase can be obtained by combining and collecting the eluate of the component peaks according to the inhibitory effect and freeze-drying. The present invention provides an active component that is isolated from soybean fermented products and clearly has an inhibitory effect on α-glucosidase, and provides a new idea for the industrial preparation and application of α-glucosidase inhibitors derived from soybean fermented products. The obtained α-glucosidase Glucosidase inhibitors can be directly used in the production of products that control the absorption of carbohydrates in the intestinal tract, so as to achieve the purpose of controlling blood sugar fluctuations.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of an alpha-glucosidase inhibitor and its product and application. Background technique [0002] In recent years, diabetes has been widely prevalent all over the world, and it has become the third largest chronic disease that seriously endangers human health after tumors and cardiovascular diseases. With the rapid development of my country's economy and society, changes in dietary structure and lifestyle, and the acceleration of population aging, the prevalence of diabetes is also showing a trend of rapid increase. While the prevalence of diabetes is rising rapidly, the awareness rate, treatment rate and control rate of diabetes in my country are obviously low. Diabetes has become a public health problem that seriously endangers the health of the population in our country, and has an increasingly serious impact on economic and social development. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/00A23L11/00A61K45/06A61K36/48A61P3/10A61P3/02A23L11/50
CPCC12P1/04A23V2002/00A61K36/00A23V2400/169A23V2200/30
Inventor 韩瑨郭本恒吴正钧刘欢欢
Owner BRIGHT DAIRY & FOOD CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap