A method for breeding offspring of calyx arachnids by cultivating immature seeds
An immature, cobweb calyx technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as cobweb calyx reproduction difficulties, research reports, and seeds that cannot germinate normally, so as to save rapid propagation time and pollution rate Effect of low and increased reproduction levels
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[0022] Materials: In this example, immature seeds of calyx arachnids were used as explants for culture.
[0023] Step (1): Collect materials
[0024] The immature fruits of calyx cobwebs were collected in Fengyang Mountain Nature Reserve from mid-to-late September to early October. The color is light green, and the top hole is closed.
[0025] Step (2): Material disinfection
[0026] Soak the picked immature fruit in detergent water (add 5ml of detergent to 1L of water) and wash it for 20 minutes, rinse it with tap water for 2 hours, and then operate it on an ultra-clean workbench with a volume fraction of 75%. Soak in ethanol for 30 seconds, wash with sterile water 3 times, each time for 1 minute, then sterilize with an aqueous solution of 1 g / L mercuric chloride for 10 minutes, shake 3-5 times during this period, rinse with sterile water 5 times, each time for 1 minute , and finally blot the excess water on the explants on sterile filter paper.
[0027] Step (3): Strippin...
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