A method for breeding offspring of calyx arachnids by cultivating immature seeds

An immature, cobweb calyx technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as cobweb calyx reproduction difficulties, research reports, and seeds that cannot germinate normally, so as to save rapid propagation time and pollution rate Effect of low and increased reproduction levels

Active Publication Date: 2016-05-18
ZHEJIANG UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

It was found that in the two methods, the mature seeds could not germinate normally, indicating that it is very difficult for the calyx to use seeds to reproduce
Therefore, there is an urgent need for a complete set of technologies and rapid propagation systems that can improve the seed reproduction of the calyx to rescue and multiply this endangered plant, but so far there is no research report on this aspect

Method used

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  • A method for breeding offspring of calyx arachnids by cultivating immature seeds
  • A method for breeding offspring of calyx arachnids by cultivating immature seeds
  • A method for breeding offspring of calyx arachnids by cultivating immature seeds

Examples

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Embodiment 1

[0022] Materials: In this example, immature seeds of calyx arachnids were used as explants for culture.

[0023] Step (1): Collect materials

[0024] The immature fruits of calyx cobwebs were collected in Fengyang Mountain Nature Reserve from mid-to-late September to early October. The color is light green, and the top hole is closed.

[0025] Step (2): Material disinfection

[0026] Soak the picked immature fruit in detergent water (add 5ml of detergent to 1L of water) and wash it for 20 minutes, rinse it with tap water for 2 hours, and then operate it on an ultra-clean workbench with a volume fraction of 75%. Soak in ethanol for 30 seconds, wash with sterile water 3 times, each time for 1 minute, then sterilize with an aqueous solution of 1 g / L mercuric chloride for 10 minutes, shake 3-5 times during this period, rinse with sterile water 5 times, each time for 1 minute , and finally blot the excess water on the explants on sterile filter paper.

[0027] Step (3): Strippin...

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Abstract

The invention discloses a method for culturing and propagating descendants of platycrater arguta through immature seeds, which aims at solving the problem of underdeveloped seed propagation of rare platycrater arguta of national secondary protection. The method is that the immature fruits of platycrater arguta are collected under the natural condition; the surfaces of the fruits are sterilized, then the immature seeds and some ovary tissue are picked off to propagate the descendants of platycrater arguta by the culture technology, so as to obtain more sterile seedlings of platycrater arguta. The method has the advantages that the explants are the immature seeds which are cultured through an artificial medium, and therefore, the defect of undeveloped propagating of the seeds can be reduced, and more platycrater arguta plants can be obtained within a short period. According to the method, the operability is high, simple and practical effects are achieved, the pollution rate is low, and the method has important significance on storage and quick propagation of rare plants.

Description

technical field [0001] The invention relates to a method for cultivating and breeding offspring of calyx arachnids by cultivating immature seeds, and relates to the technical field of achieving the purpose of preserving and propagating rare species by improving the breeding level of seeds. Background technique [0002] Cobweb calyx is a plant of the genus Platycrater. It is a small deciduous shrub that grows in the evergreen forests of low mountains and hills at an altitude of 400-1000 meters. It likes to grow beside streams and damp bare rocks. Bad, 0.5-1 meter high, flowering in July, fruit ripening in October, many seeds, dark brown. It is a single species endemic to East Asia, distributed intermittently in China and Japan, and distributed in Zhejiang, Anhui, Jiangxi, Fujian and other places in China. In addition to being used as an ornamental, it also has important scientific value for the study of plant geography and flora. [0003] This species has a narrow distribut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈利萍李俊星叶立新邹晓霞刘胜龙
Owner ZHEJIANG UNIV
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