A method of preserving the offspring of a very small population of Vaccinium vaccinium through immature seeds

Abstract

The invention discloses a method for preserving minimum population Guangxi bilberry offspring by means of immature seeds.The problem that minimum population Guangxi bilberries in Zhejiang province can not be reproduced easily under the natural condition is solved.According to the method, immature fruits of the Guangxi bilberries are collected in a natural protecting area, after the surfaces of the fruits are sterilized, immature seeds and a part of ovary tissue are picked off, by means of the tissue cultivation technology, the Guangxi bilberry offspring is bred, and more Guangxi bilberry sterile seedlings are obtained within a short time.The method has the advantages that adopted explants are the immature seeds, an artificial culture medium is utilized for cultivating the explants, the defect that under the natural condition, seed propagation is not developed can be overcome, and more Guangxi bilberry plants can be obtained within a short term.The method is high in operability, simple, practical and low in pollution rate, and has great significance for preservation and propagation of minimum populations.

Description

technical field [0001] The invention relates to a method for preserving the offspring of a very small population of Guangxi vaccinia through immature seeds, and relates to the technical field of achieving the purpose of preserving and multiplying a very small population by improving the seed breeding level. Background technique [0002] Guangxi Vaccinium belongs to Rhododendronaceae Vaccimium (Vaccimium) plant, is an evergreen shrub, born in the forest at an altitude of 1200-1700 meters, on rocks in valleys or epiphytic on the trunk of Fagaceae. Guangxi bilberries are mainly distributed in Hunan, Guangdong and Guangxi. In 2003, the "Fengyang Mountain Rare Plants and Vegetation Survey" first discovered and reported the distribution of Guangxi bilberry in Zhejiang Province, but its quantity is rare, with less than 50 plants distributed in the whole province, and it was included in the list of extremely small population protection in Zhejiang Province . At present, the common...

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Problems solved by technology

Therefore, there is an urgent need for a complete set of technologies that can improve the reproductive level of Vaccinium vaccinium to preserve Vaccinium custardum and prevent the extremely small population of Vaccinium vaccinia from becoming extinct due to the difficulty in breeding under natural conditions, but so far there is no report on this aspect

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