Preparation method of photosynthetic bacterial manure

Abstract

The invention provides a preparation method of photosynthetic bacterial manure, and particularly provides the preparation method of photosynthetic bacterial manure for preventing and controlling hydrogen sulfide poisoning of rice. According to the preparation method, a thiocapsa roseoppersicina strain in purple sulfur photosynthetic bacterium is adopted and treated by steps of semi-solid seed activated culture, seed culture, illumination culture in an anaerobic fermentation tank and the like to obtain high-concentration bacterium liquid of the thiocapsa roseoppersicina; the high-concentration bacterium liquid can be capable of rapidly degrading hydrogen sulfide in a rice field, promoting root development of the rice and restoring soil so as to play an role in increasing the yield. According to the preparation method disclosed by the invention, the prescription of a culture medium for culturing the thiocapsa roseoppersicina and the culture condition are optimized a lot of times, so that the culture time is greatly shortened, 2 thousand of million cfu / ml of photosynthetic bacterium thiocapsa roseoppersicina bacterial manure can be obtained just after 7-10 days of fermentation, the production efficiency is improved and the production cost is lowered. In the photosynthetic bacterial manure disclosed by the invention, the thiocapsa roseoppersicina is high in hydrogen sulfide degrading speed and low in used amount for a large field; the effect of increasing the yield can be achieved by applying 100ml of the photosynthetic bacterial manure per mu every time.

Description

technical field [0001] The invention belongs to the technical scope of functional microbial bacterial fertilizer, and in particular relates to a preparation method of photosynthetic bacterial fertilizer for preventing and treating rice hydrogen sulfide poisoning. Background technique [0002] Sulfur is a constituent of sulfur amino acids such as methionine and cysteine. In addition to the organic state of sulfur in plants, a considerable amount of sulfur exists in the inorganic state and participates in redox reactions. Sulfur is also a component of chlorophyll, but when lacking, the stems and leaves turn yellow, so it can be considered that sulfur is indirectly involved in the production of chlorophyll. The movement of sulfur in the body is similar to that of nitrogen and phosphorus. After being absorbed by rice, it moves from the stem to the sheath and leaves, and moves to the ear after the leaves synthesize proteins. The content of sulfur in stems and leaves is similar ...

AI Technical Summary

Problems solved by technology

The researchers believe that these purple non-sulfur bacteria cannot use sulfide or can only be used when the concentration of sulfide is kept at a very low value, or that the presence of purple non-sulfur bacteria induces other sulfur-oxidizing bacteria growth to reduce hydrogen sulfide content (Xiuzhu Li), in other words, the direct degradation of sulfide by its purple non-sulfur bacteria is insignificant or insignificant

However, the degradation effect of purple sulfur bacteria on sulfide is significant, but there are few studies. Feng Su of Sichuan University has conducted research on Sulfur japonica among the purple sulfur bacteria. Fertilizers and treatment of sulfur pollutants

But to put it into practical use, the first problem to be solved is that the growth rate of the bacteria is slow, and it usually takes two weeks to cultivate for a clear growth trend. It can be used as a bacterial agent after 20-40 days of cultivation, and the value of OD650 Not higher than 1, the live bacteria content is lower than 100 million cfu / ml, and the long growth cycle is not conducive to large-scale application

And up to the present, also do not see relevant technical scheme of preparation method of Sulfur persicae to disclose