Pseudomonas aeruginosa strain for degrading pesticides containing ester bonds

A technology of Pseudomonas aeruginosa and strains, applied in the field of environmental microorganisms and agriculture, can solve the problem of no ester bond-containing pesticides, etc., and achieve the effects of strong environmental adaptability, convenient use, and high degradation efficiency

Active Publication Date: 2015-06-03
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are currently no microorganisms and biodegrada

Method used

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  • Pseudomonas aeruginosa strain for degrading pesticides containing ester bonds
  • Pseudomonas aeruginosa strain for degrading pesticides containing ester bonds
  • Pseudomonas aeruginosa strain for degrading pesticides containing ester bonds

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0023] Example 1 Enrichment culture of bacterial strain PAO1

[0024] The present inventors repeatedly screened and rejuvenated the wild-type strain PAO1 by enrichment culture method, so that it has the ability of high drug resistance and stable degradation. The medium used for enrichment culture is basal medium (the formula is: (NH 4 ) 2 SO 4 , 2 g; MgSO4, 0.2 g; CaCl 2 , 0.01 g; FeSO 4 , 0.005 g; MnCl 2 , 0.002 g; K 2 HPO 4 , 10.5 g; KH 2 PO 4 , 4.5 g, 1000 mL distilled water, pH 7.5). At the same time, the mother solution of beta-cypermethrin (DMSO as the solvent) was added to make the initial mass concentration of beta-cypermethrin 50 μg / mL, and the enrichment culture was carried out at 30 °C and 150 r / min on a shaking table. After 7 days of culture, 10% of the inoculum was transferred to the second batch of basal medium containing 100 μg / mL beta-cypermethrin. After 7 days of culture under the same conditions, 10% of the inoculum was transferred to the basal med...

Embodiment 2

[0027] Example 2 Physiological and biochemical characteristics of bacterial strain PAO1

[0028] The Pseudomonas aeruginosa ( Pseudomonas aeruginosa ) strain PAO1 grew faster on the LB solid plate, and the colony was yellow-green, with a raised center and flat edges. Through the determination of physiological and biochemical characteristics, it was shown that the strain belonged to Gram-negative bacilli, aerobic, positive for oxidase, gelatin liquefaction, and nitrate reduction reaction; negative for indole reaction. The bacterium can decompose and utilize glucose and xylose; it does not decompose and utilize sucrose and lactose.

Embodiment 3

[0029] Example 3 Pesticide Degradation Test by Bacterial Strain PAO1

[0030] Beta-cypermethrin, lambda-cyhalothrin, deltamethrin, cypermethrin, fenpropathrin, bifenthrin, kysstrobin, pyraclostrobin and Azoxystrobin, so that the final concentration was 100 μg / mL; the inoculum volume was 0.2 g / L, and the culture medium without inoculation was used as the control, cultured at 30 °C for 0-5 days, samples were taken regularly, and the pesticide concentration was detected by HPLC. Residual amount and calculation degradation rate, the results are shown in Table 1. The results showed that the strain PAO1 could effectively degrade lambda-cypermethrin, lambda-cyhalothrin, deltamethrin, cypermethrin, fenpropathrin, bifenthrin, kresstrobin, pyraclostrobin and pyrimidine in a short time. Strostrobin, the degradation rates of the above pesticides reached 91.4%, 86.1%, 84.5%, 82.8%, 70.2%, 52.2%, 87.7%, 86.3% and 68.0% within 5 days, respectively, showing good biodegradation effect.

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Abstract

The invention belongs to the field of microorganisms and agriculture of the application environment and particularly relates to a pseudomonas aeruginosa strain PAO1 for degrading pesticides containing ester bonds. The collection number of the strain is CCTCC M 2014630. The strain is wide in degradation spectrum, and can be used for degrading the pesticides containing the ester bonds such as high-efficiency cypermethrin, high-efficiency cyhalothrin, deltamethrin, cypermethrin, fenpropathrin, bifenthrin, kresoxim-methyl, pyraclostrobin and azoxystrobin. The strain is strong in environmental adaptability, good in degrading effect and convenient in use, can be used for degrading the pesticides containing the ester bonds, and has very important application value in the aspects of removing pesticide residues in the environments such as water bodies and soil.

Description

technical field [0001] The invention relates to the fields of applied environmental microorganisms and agriculture. More specifically, it relates to a strain of Pseudomonas aeruginosa that degrades pesticides containing ester bonds. Background technique [0002] The large and frequent use of pesticides has brought serious harm to the ecological environment and human health, especially the pesticide residue pollution caused by the widespread use of pyrethroid insecticides and methoxyacrylate fungicides cannot be ignored. These two types of pesticides are pesticides containing ester bonds in structure, and are the main types in the pesticide market at present. A large number of studies have shown that these two types of pesticide residues not only cause great harm to non-target organisms such as bees, fish, and aquatic invertebrates, but also have chronic toxicity to humans and other mammals. Long-term exposure to or ingestion of these two types of pesticides Pesticides will...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/385
CPCB09C1/10C02F3/34C02F2101/306C12N1/205C12R2001/385
Inventor 张炼辉陈少华常长青邓音乐刘诗胤
Owner SOUTH CHINA AGRI UNIV
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