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Comprehensive antioxidant performance evaluation method of antioxidants
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A technology for comprehensive evaluation of antioxidant capacity, applied in the field of chemical analysis, can solve problems such as the antioxidant activity of unformed antioxidants
Inactive Publication Date: 2015-06-17
JIANGNAN UNIV
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However, a unified or standard evaluation method for the antioxidant activity of antioxidants has not yet been formed.
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Embodiment 1
[0023] Example 1: Put V E , V C , citric acid and TBHQ form a composite antioxidant, specifically using U in Table 1 12 (12 10 ) uniform design table to arrange experiments.
[0026] The uniform test results are shown in Table 2.
[0027] Table 2 Uniform test results
[0028]
[0029] The ability to scavenge hydroxyl radicals in Table 2 (S 1 ), the ability to scavenge superoxide anion (S 2 ), lipid oxidation inhibitory ability (S 3 ) and the data of the comprehensive evaluation index S are used for regression calculation, and the regression equation is obtained:
[0030] S=-66.9-202A+12.9B+101C+288D
[0031] In the above formula, A, B, C, D are V E , V C , the amount of citric acid and TBHQ added.
[0032] The analysis of variance is shown in Table 3. When significant level α=0.05, F=2.630.05 ); at significant level α=0.10, F=2.630.10 ). It shows that the above regression equation is not credible a...
Embodiment 2
[0037] Example 2: Put V E , V C , citric acid, TBHQ, BHT and BHA form a composite antioxidant, specifically using the U of Table 4 13 (13 12 ) uniform design table to arrange experiments.
[0040] The uniform test results are shown in Table 5.
[0041] Table 5 Uniform test results
[0042]
[0043] The ability to scavenge hydroxyl radicals in table 5 (S 1 ), the ability to scavenge superoxide anion (S 2 ), lipid oxidation inhibitory ability (S 3 ) and the data of the comprehensive evaluation index S are regressed using Minitab software, and the regression equation is obtained:
[0044] S=1.76-20.3A-16.1B+36.2C-1.83D-3.48E+5.13F
[0045] In the above formula, A, B, C, D, E, F are TBHQ, BHT, BHA, V E , V C and the amount of citric acid added.
[0046] The variance analysis of JN4 is shown in Table 6. At the significant level α=0.05, F=4.60>F 0.05 (6,6)=4.28, so the regression equation is credible at th...
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Abstract
The invention relates to a comprehensive antioxidant performance evaluation method of antioxidants. The hydroxyl group eliminating performance (S1), the superoxide anion eliminating performance (S2), and the grease inhibiting performance (S3) are taken as the indexes, and a comprehensive evaluation index (S) is defined as: S=S1+S2+S3. The relationships between the comprehensive evaluation index (S) and the evaluation indexes (S1, S2, and S3) are established through uniform design and a regression analysis method. If the S value namely comprehensive score is high, the result shows that the synergetic effect is strong after antioxidants are compounded, and the antioxidant effect is very good. If the S value is low, and the antioxidant effect is bad. In the provided method, a plurality of methods for evaluating antioxidant performance of antioxidants are organically combined, thus the effects and contributions of each antioxidant in a compounded antioxidant can be conveniently and effectively analyzed, and useful references are provided for the design of compounded antioxidants and evaluation on overall antioxidant effect.
Description
technical field [0001] The invention relates to a comprehensive evaluation method for antioxidant capacity and belongs to the technical field of chemical analysis. Background technique [0002] Antioxidants are widely used in many fields, but so far there is no accurate definition. Antioxidants are not only considered to be special oxidative enzymes with inhibitory effect, substances that can preferentially react with oxidants that cause body damage, but also substances that can isolate metal ions or have some ability to repair the system. At present, there is no unified standard for the evaluation of antioxidant activity, so there is no so-called "best" antioxidant. This requires a comprehensive evaluation of the antioxidant capacity of antioxidants from multiple angles or using multiple methods. [0003] Although there is no unified standard for the evaluation of antioxidant activity, there are two basic evaluation methods: lipid peroxidation evaluation method and free r...
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