Method for producing structured microcarriers
A microcarrier and three-dimensional structure technology, applied in the field of microcarriers, can solve the problems of disrupting the uniform distribution, disrupting the uniformity of the fluid velocity, affecting the reliability of the assay, and achieving the effect of facilitating processing and enhancing mechanical properties.
Inactive Publication Date: 2015-06-17
麦卡提斯有限责任公司
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Problems solved by technology
[0021] Furthermore, laminar flow is disturbed by improperly oriented microcarriers1
In fact, in this case, the laminar flow is forced to move around the microcarrier 1 involved, thus causing the uniformity of the fluid velocity along the microchannel 10 to be disturbed, thereby disturbing the interaction with the microcarrier 1. Uniform distribution of reagents and target molecules interacting with the detection surface5
This affects the reliability of the assay
Method used
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Embodiment Construction
[0065] will refer to Figures 6 to 17 A method for producing microcarriers according to the invention is described. The method comprises the following sequential steps.
[0066] like Image 6 As shown, the first step involves providing a wafer 15 having a sandwich structure comprising a bottom layer 16, a top layer 17 and an insulating layer 18 between the bottom and top layers 16,17.
[0067] For example, the wafer 15 is an SOI (silicon on insulator) wafer with a diameter of 100 mm, a bottom layer 16 of 380 μm thickness, an insulating layer 18 of 1 μm thickness and a top layer 17 of 10 μm thickness. The top layer 17 and the bottom layer 16 are made of single crystal silicon, and the insulating layer 18 is made of silicon dioxide.
[0068] The method according to the invention further comprises the step of structuring the top layer 17 to define a three-dimensional structure on the top surface 19 of the top layer 17 .
[0069] For this purpose, the second step involves appl...
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Abstract
The present invention relates to a method for producing microcarriers (1), the method comprising the steps of providing a wafer having a bottom layer (16), a top layer and an insulating layer (18), structuring the top layer to define at least one three-dimensional structure (7a) on the top surface of the top layer, etching away the top layer to delineate lateral walls (22) of bodies (6) of the microcarriers, applying a continuous polymer layer over the top surface (4) of the bodies (6) of the microcarriers, removing the bottom layer (16) and the insulating layer (18), structuring the bottom surfaces of the bodies (6) of the microcarriers to define at least one three-dimensional structure on the bottom surface of each body, and removing the polymer layer to release the microcarriers.
Description
technical field [0001] The present invention relates to a microcarrier and a method for producing the microcarrier. In particular, the present invention relates to microcarriers suitable for performing biological and / or chemical assays for research and clinical experiments. [0002] Within the scope of the present invention, microcarriers or microparticles refer respectively to particles of any type, carriers of any type, which are microscopic in size, generally having a maximum dimension of 100 nm to 300 μm, preferably 1 μm to 200 μm. [0003] According to the present invention, the term microcarrier refers to functionalized or adapted to be functionalized microparticles comprising or adapted to comprise more than one Ligands or functional units. A large spectrum of chemical and biological molecules can be attached to microcarriers as ligands. Microcarriers can have multiple functions and / or ligands. The term functional unit as used herein is meant to define any species t...
Claims
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IPC IPC(8): B81C1/00G01N15/14G01N33/543B01L3/00
CPCB01L3/502707B01L3/502761B01L3/545B01L2200/0647B01L2200/10B01L2200/12B01L2300/0803B01L2300/0819B01L2300/0893G01N15/1456B01L2300/16
Inventor 尼古拉斯·德米埃尔斯蒂芬·甘佩尔拉斐尔·托尔奈菲利浦·雷诺
Owner 麦卡提斯有限责任公司