Hibiscus rosa-sinensis flower bud extracts and application thereof

A technology of extract and hibiscus flower, applied in the field of plant extracts and their therapeutic uses, can solve the problems of complex ingredients and uneven effects, and achieve the effects of good wound shrinkage, enhanced immunity, and antibacterial immunity.

Inactive Publication Date: 2015-06-24
SHANTOU UNIV MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In various products for furuncle carbuncle and repairing problems, the addition of medicinal materials or related ingredients such as Coptis chinensis, Scutellaria baicalensis, Cortex Phellodend

Method used

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  • Hibiscus rosa-sinensis flower bud extracts and application thereof
  • Hibiscus rosa-sinensis flower bud extracts and application thereof
  • Hibiscus rosa-sinensis flower bud extracts and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Preparation of hibiscus flower bud extract

[0028] Ethanol extract of hibiscus flower: pick the buds of fresh hibiscus flower, use 70%-90% ethanol to heat and extract, the ratio of solid to liquid is 1:7 to 1:10, heat and extract three times, respectively 60min, 30min, 30min, and suction filter three times The extracts are combined, concentrated on a rotary evaporator at 45°C, transferred to an evaporating dish in an oven, and dried at 40°C to 50°C until the extract is dried to obtain the total ethanol extract extract of hibiscus flower buds;

[0029] Preparation of n-butanol part extract and ethyl acetate part extract: prepare the obtained extract extract with distilled water to a water extract solution with a concentration of 20mg / ml to 50mg / ml, and use n-butanol and ethyl acetate to extract respectively , the ratio of water to organic reagent is 1:1 to 1:2, extract 8 to 10 times, combine the extracts of organic solvents, place them on a rotary evaporator a...

Embodiment 2

[0031] Example 2 Model used in the study of hibiscus bud extract

[0032] Boil and carbuncle model: experiment with KM mice, back shaving machine hair removal, use alcohol to wipe the surface, use 10% chloral hydrate to anesthetize the mice, dose 400mg / kg; prepare turpentine mixture of 0.2% croton oil (0.2ml bar Soybean oil was dissolved in 100ml turpentine and ultrasonically mixed), and intradermal injection was used, 0.04-0.1ml each, depending on the severity of the required model. Criteria for observation results: boils and carbuncle pustules, hard scabs, thick and hard scabs after pus ulceration, scab removal, and normal skin after scab removal.

[0033] Full-thickness resection model: KM mice were used for the experiment, the back was shaved, disinfected with alcohol, anesthetized with 10% chloral hydrate at a dose of 400 mg / kg, and the skin on the back of the mouse was punched with a stainless steel puncher with a diameter of 5 mm, deep to the fascia. Observation crite...

Embodiment 3

[0034] Example 3 The medicinal effect of the extract of hibiscus bud n-butanol

[0035] On the basis of the research on the water extract of hibiscus flower buds, the same method was used to compare the water extract of hibiscus flower buds with the ethanol extract, and the medicinal effects were basically the same. However, considering the advantages of alcohol extraction in filtration, concentration, drying and preservation, the method used Continue to study the alcohol extraction part.

[0036] The furuncle carbuncle model was used to verify the efficacy of the n-butanol and ethyl acetate parts of the hibiscus flower buds, and the positive drug was Bactobang. Take medicine for about 9 days, use the drug concentration of 80mg / ml-150mg / ml, use the solvent 4% DMSO, set the positive group and the solvent blank control group at the same time; observe the condition of the wound during the medication, record the reduction of pustules and the formation of hard scabs , and indicato...

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Abstract

The invention relates to hibiscus rosa-sinensis flower bud extracts and therapeutic application thereof and particularly relates to application of hibiscus rosa-sinensis flower bud extracts to preparation of antibacterial medicines. The hibiscus rosa-sinensis flower bud extracts are collected from the n-butanol extraction section and the ethyl acetate extraction section after being extracted with ethanol, n-butanol and ethyl acetate in sequence, or the hibiscus rosa-sinensis flower bud extracts are collected from the 30% ethanol solution extraction section and the 60% ethanol solution extraction section after being extracted with the ethanol solution and eluted by using an AB-8 column in water, the 30% ethanol solution, the 60% ethanol solution and the 95% ethanol solution in sequence. By using the hibiscus rosa-sinensis flower bud extracts provided by the invention, the wound can heal quickly and contract well, and the crust can fall away from skin basically.

Description

technical field [0001] The invention relates to a plant extract and its therapeutic application, in particular to the application of hibiscus flower bud extract in the treatment of skin diseases. Background technique [0002] Furuncle carbuncle is explained by modern medicine as the infection of skin hair follicles, sweat glands and other tissues caused by germs, resulting in redness, swelling and pustules. It can be found on the head and face, neck, back, buttocks, armpits and perineum. According to the severity of the disease, you can use disinfectants such as alcohol to clean, or use antibiotics for internal or external use, and in severe cases, drainage and medication are required; if you use traditional medical methods, you can use Chinese herbal medicines with related effects for internal and external washing. Can achieve better results. However, mild boils, especially mild boils that appear on the face, are often called small boils or acne on women's faces. These ar...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P17/00A61P31/04A61P37/04A61K133/00
Inventor 石刚刚姜霁洋
Owner SHANTOU UNIV MEDICAL COLLEGE
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