Purification method of lysine fermentation liquid
A technology of lysine fermentation broth and purification method, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of large consumption of raw materials, high cost, shortening of extraction process routes, etc., to reduce extraction cost, reduce the consumption of raw and auxiliary materials, and shorten the effect of the extraction process route
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[0026] As long as the inventive method ensures that the lysine content in the lysine fermentation broth is 130-180 grams per liter, SO 4 2- The content of lysine is ≤ 8 g / L, and there is no special requirement for the preparation method of such lysine fermentation liquid. For example, the preparation method may include: under the condition of producing lysine, inoculating lysine fermentation bacteria into the lysine fermentation medium, and under the condition of feeding carbon source and feeding nitrogen source, the fermentation culture is carried out, and the nitrogen source includes ammonium chloride.
[0027] Preferably, the amount of feeding the carbon source makes the concentration of reducing sugar in the medium during the fermentation process controlled at 5-10 grams per liter, and the amount of feeding the nitrogen source makes the concentration of nitrogen in the medium during the fermentation process controlled at 0.45-0.95 g / L. The concentration of nitrogen is 0....
Embodiment 1
[0068] This example is used to illustrate the purification method of the lysine fermentation broth provided by the present invention.
[0069] (1) Machining 100 parts by weight of harvested corn to pulverize corn grains so that the passing rate of corn flour through a 30-mesh sieve is 80%.
[0070](2) Mix the pulverized product with water to 12Bé o , relative to the dry weight per gram of the pulverized product, 20 units of enzyme activity units of amylase (α-amylase, Novozymes) were added, and enzymolysis was carried out at 90°C and pH 5.5 for 100 minutes to obtain enzymolysis product. Among them, the enzymatic hydrolysis product is filtered through a hydraulic plate and frame filter press to separate the enzymatic hydrolysis liquid (solid content is 20% by weight); after that, 115 enzyme activity units of glucoamylase (α-1,4 - Glucose hydrolase, Novozymes), enzymatically hydrolyze for 420 minutes under the conditions of 60°C and pH 4.5 to obtain the saccharification liquid...
Embodiment 2
[0076] This example is used to illustrate the purification method of the lysine fermentation broth provided by the present invention.
[0077] The preparation method of starchy raw material saccharification liquid, the formula of seed tank medium, and the method of culturing mature seed liquid in seed tank are the same as in Example 1.
[0078] Use the saccharification clear liquid of the starchy material obtained to prepare the fermentation medium, with respect to every liter of fermentation medium, the consumption of ammonium sulfate is 15 grams, the consumption of magnesium sulfate is 0.15 grams, other components and their consumptions are all the same as those in Example 1. same. The culture medium was heated to 121°C for 30 minutes, then cooled to 31°C and kept constant, and the pH was adjusted to 6.9 with ammonia water. Determination of SO in the prepared fermentation medium (ie initial medium) 4 2- The content is 11.03 g / L.
[0079] The medium volume in the fermente...
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