Molecular markers for various traits in wheat and methods of use

A wheat, marker gene technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, applications, etc., can solve a lot of time, money and other problems

Inactive Publication Date: 2015-06-24
PIONEER HI BRED INT INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Furthermore, because these traits are influenced by environmental conditions, wheat lines need to be phenotyped at several locations and over several years, requiring significant time, monetary, and land resources

Method used

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  • Molecular markers for various traits in wheat and methods of use
  • Molecular markers for various traits in wheat and methods of use
  • Molecular markers for various traits in wheat and methods of use

Examples

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example

[0503] The following examples are offered to illustrate, but not limit, the appended claims. It should be understood that the examples and embodiments described herein are for illustrative purposes only, and that those skilled in the art will recognize that various changes can be made without departing from the spirit of the invention or the scope of the appended claims. or parameters.

example 1

[0505] Genome-wide association studies

[0506] An association mapping strategy was employed to identify markers in wheat associated with anthesis, heading, anther extrusion, and head blight resistance. In the association analysis described above, a collection of 240 wheat lines was analyzed by evaluating SNPs at approximately 3,100 loci. SNP information was obtained using the Illumina Infinium HD assay. The above-mentioned lines cover elite germplasm, commercially launched cultivars, and other published varieties.

[0507] Phenotypic scores were obtained for anthesis, heading, anther extrusion, and head blight resistance in wheat using procedures familiar to those of ordinary skill in the art.

[0508] Phenotype scores and marker information for each of the above 240 lines were entered into the association analysis. Structure-based association analyzes were performed using standard association mapping methods, where population structure was controlled for using labeled d...

example 2

[0578] Identification of favorable and unfavorable marker alleles

[0579] Wheat lines used in association studies can be sorted by phenotype and evaluated at each marker locus found to be associated with the corresponding phenotype. For anthesis, this included any of the marker loci disclosed in Table 1. For heading date, this included any of the marker loci disclosed in Table 2. For head blight resistance this included any of the marker loci disclosed in Table 3. For anther extrusion this included the markers disclosed in Table 4. Alleles at each marker locus associated with a favorable phenotype (or, unfavorable phenotype) can then be identified.

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Abstract

This disclosure relates to methods of identifying and / or selecting wheat plants or germplasm by detecting markers associated with flowering date, anther-extrusion, heading date and / or fusarium head blight resistance.

Description

[0001] Indexes to sequence listings are submitted as text files via EFS-WEB [0002] The official version of the sequence listing is submitted as a text file through EFS-Web at the same time as this description, which conforms to the American Standard Code for Information Interchange (ASCII), the file name is 435138seqlist.txt, and the creation date is 2013 On July 9, the size is 156Kb. The sequence listing submitted via EFS-Web is part of this specification and is hereby incorporated by reference in its entirety. technical field [0003] The present disclosure relates to compositions and methods for identifying and / or selecting wheat plants or germplasm for heading, anthesis, anther extrusion, and / or head blight resistance. Background technique [0004] Wheat is one of the most important crops worldwide. Worldwide demand for wheat requires continuous improvements in wheat yield and quality. While conventional wheat breeding has achieved increases in wheat yield in the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01H5/10C12Q1/68
CPCA01H5/10C12Q2600/156C12Q1/6895A01H6/4678A01H1/045
Inventor W.拉斯卡S.鲁克A.桑德胡P.沃特斯
Owner PIONEER HI BRED INT INC
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