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A kind of metal complexing method for separating and purifying sagebrush flavonoids

A technology of separation and purification and metal complexation, applied in the field of separation and purification of sage flavonoids, can solve the problems of difficult industrialization, long production cycle, high purification cost, etc., and achieve the effect of easy implementation, low cost and good separation effect

Inactive Publication Date: 2018-03-09
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research and reports on the separation and purification process of various plant flavonoids are common. At present, the common separation and purification methods for plant flavonoids include column chromatography, membrane separation, macroporous resin adsorption, high-efficiency countercurrent chromatography, and high-efficiency preparation solution. Phase chromatography, molecular imprinting separation method, etc. These methods generally have the disadvantages of long production cycle, difficult operation, high purification cost, and difficulty in industrialization. Therefore, it is necessary to find a fast, safe, and feasible method for effectively separating and purifying plant flavonoids. The method is one of the important topics in the study of flavonoids today

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Extraction of flavonoids from Salmonella: Accurately weigh 10 kg dried 100-mesh Salmonella powder, add a certain amount of 70% ethanol solution at a ratio of 1:35 to infiltrate for about 30 minutes, and place it in an ultrasonic power of 250 W, extraction temperature is 35 ℃ in ultrasonic equipment for 60 min, and combine the extracts;

[0026] 2) Concentration under reduced pressure: filter the extract with suction and concentrate by rotary evaporation to about 5% of the original volume;

[0027] 3) Removal of polysaccharides and proteins: Dissolve with absolute ethanol at a ratio of 1:9 and let stand for 12 hours to precipitate polysaccharides and proteins, filter with suction, concentrate in vacuo, and freeze-dry to obtain a solid powder of crude extract of sagera flavonoids.

[0028] 4) Separation and purification: Weigh 1 kg of the solid powder of the crude extract of S. sagerum flavonoids, and use absolute ethanol to prepare a 1.5 mg / mL sample solution of S. sa...

Embodiment 2

[0030] 1) Extraction of flavonoids from Salmonella: Accurately weigh 5 kg dried 100-mesh Salmonella powder, add a certain amount of 75% ethanol solution as the extractant at a ratio of 1:15 to infiltrate for about 60 minutes, and place the ultrasonic power at 450 W, extraction temperature is 45 ℃ ultrasonic equipment for 45 min, combined extract;

[0031] 2) Concentration under reduced pressure: filter the extract with suction and concentrate by rotary evaporation to about 5% of the original volume;

[0032] 3) Removal of polysaccharides and proteins: Add absolute ethanol at a ratio of 1:4 to dissolve and let stand for 12 hours to precipitate polysaccharides and proteins, filter with suction, concentrate in vacuo, and freeze-dry to obtain a solid powder of the flavonoid crude extract of S. sativa.

[0033] 4) Separation and purification: Weigh 0.50 g of the solid powder of the flavonoid crude extract of S. sageris, and prepare it with absolute ethanol to prepare a flavonoid sa...

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Abstract

The invention relates to a method for separating and purifying flavonoids in the industries of food, health care products, cosmetics and pharmaceuticals, in particular to a method for separating and purifying flavonoids from sagebrush. The present invention adopts the metal salt solution to react with the crude extract of sage flavonoids to form sage flavonoid metal complexes, and the latter undergoes the dissociation of ethylenediaminetetraacetic acid to separate the sage flavonoids from the metal complexes. Dissociated from the compound, so as to realize the separation and purification of sage flavonoids. The method has the advantages of easy operation, good separation effect, low cost, short cycle, etc., and can break through the technical bottleneck restricting the industrial separation and purification of flavonoid compounds. This process can provide theoretical reference for the efficient utilization of sage flavonoids and the development of related functional foods and medicines, and has important practical significance for the comprehensive utilization and development of wild resources, as well as the improvement of social and ecological benefits. The process is safe and reliable, and is also applicable to the separation and purification of various other flavonoids.

Description

technical field [0001] The invention relates to a method for separating and purifying flavonoids in the industries of food, health care products, cosmetics and pharmaceuticals, in particular to a method for separating and purifying flavonoids from sagebrush. Background technique [0002] Salmonella is the whole plant of Compositae. It is mainly wild and widely distributed. It can grow in most parts of the country, such as East China, Central South, Southwest, Hebei, Shaanxi, Taiwan and other places, and is rich in resources. It is sweet and flat in nature and flavor, and has the effects of eliminating throat fire, relieving heat, detoxifying, regulating middle-Jiao Qi, stopping diarrhea, eliminating phlegm, suppressing qi, and removing heat and cough. It can be used for colds, coughs, bronchitis, and asthma , high blood pressure, broad bean disease, rheumatism, low back pain and other treatment; external treatment of bruises, snake bites. Relevant studies have found that sa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/28A61K8/9789A23L33/105
Inventor 吕峰林华婷黄国钞李燕妹汤宇青
Owner FUJIAN AGRI & FORESTRY UNIV
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