Mongolian oak leaf extractive with anti-cancer effect and preparation method and application thereof

A technology of eucalyptus leaves and extracts, which is applied in the field of preparation of drugs that have an inhibitory effect on tumor cell growth, can solve problems such as experimental studies that have not been reported, and achieve the effects of simple process flow, expanded application range and value, and less investment in equipment

Active Publication Date: 2015-07-08
辽宁省农业科学院大连生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There is no report on the related experimental research on the preparation of e

Method used

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  • Mongolian oak leaf extractive with anti-cancer effect and preparation method and application thereof
  • Mongolian oak leaf extractive with anti-cancer effect and preparation method and application thereof
  • Mongolian oak leaf extractive with anti-cancer effect and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Using Quercus japonica oak leaves as raw material, the preparation method of the oak leaf extract comprises the following steps:

[0040] ①Wash the leaves with water, dry them in the shade at 25°C, crush them to 40 mesh, and extract them with deionized water at a material-to-liquid ratio of 1g / 15mL at 20°C for 12 hours each time. Use 4 layers of gauze to filter and then soak the residue Extraction, total leaching 3 times, combined extracts; centrifuged the combined extracts at 3000rpm for 10min to obtain extracts; evaporated and concentrated the extracts at 65°C to 5% of the volume of the extracts to obtain concentrates;

[0041] ② Dilute the concentrated solution obtained in step ① to 10 times the volume of the concentrated solution with deionized water, filter it at 0.05-0.08MPa in an ultrafiltration membrane device with a molecular weight cut-off of 5000Dr, and collect the extract with a molecular weight below 5000Dr.

[0042] ③ Freeze-drying the extract obtained in ...

Embodiment 2

[0044]Using oak leaves of Quercus liaotungensis as raw material, the preparation method of the extract of oak leaves is the same as in Example 1, the only difference is that step ① of this example is: wash the leaves of oak leaves with water, dry them in the shade at 25°C, and crush them to 40 mesh With 65% ethanol aqueous solution with a volume fraction of 1g / 15mL, extract at 20°C for 12 hours each time, use 4 layers of gauze to filter, then extract the residue, and extract 3 times in total. Extraction solution; the combined extraction solution was centrifuged at 3000rpm for 10min to obtain the extraction solution; the extraction solution was evaporated and concentrated at 60°C to 5% of the volume of the extraction solution to obtain the first concentrated solution, which was added to the first concentrated solution Add 10 times the volume of deionized water, evaporate and concentrate at 60°C to the volume of the first concentrated solution to obtain a concentrated solution. ...

Embodiment 3

[0046] Using oak leaves of Quercus liaotungensis as raw material, the preparation method of the extract of oak leaves is the same as in Example 1, the only difference is that step ① of this example is: wash the leaves of oak leaves with water, dry them in the shade at 25°C, and crush them to 40 mesh With 75% ethanol aqueous solution with a volume fraction of 1g / 15mL, extract at 20°C, each extraction time is 12h, use 4 layers of gauze to filter and then extract the residue, a total of 3 extractions, combined Extraction solution; the combined extraction solution was centrifuged at 3000rpm for 10min to obtain the extraction solution; the extraction solution was evaporated and concentrated to 5% of the volume of the extraction solution at 60°C to obtain the first concentrated solution, which was added to the first concentrated solution Add 10 times the volume of deionized water, evaporate and concentrate at 60°C to the volume of the first concentrated solution to obtain a concentra...

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Abstract

The invention discloses Mongolian oak leaf extractive with anti-cancer effect and a preparation method and application thereof. The preparation method includes the following steps of firstly, conducting extracting on Mongolian oak leaves through extracting agents to obtain extract liquid, and evaporating and concentrating the extract liquid to obtain a concentrated solution; secondly, adding water to thin up the concentrated solution obtained in the first step, and conducting ultrafiltration to obtain Mongolian oak leaf extract with the molecular weight smaller than or equal to 5000 Dr; thirdly, freeze-drying the extract liquid obtained in the second step to obtain freeze-dried powder, namely, the Mongolian oak leaf extractive. The experiment research on the Mongolian oak leaf extractive with the anti-cancer effect is conducted for the first time, the Mongolian oak leaf extractive fills in the blank in the field, and the prepared Mongolian oak leaf extractive can restrain growth of various cancer cells both inside and outside living bodies and has the strong anti-cancer effect. The process flow is simple, the device input is small, industrial production can be easily achieved, and no pollution is caused in the preparation process.

Description

technical field [0001] The present invention relates to an extract of eucalyptus leaves, in particular to an extract of eucalyptus leaves with an anticancer effect, a preparation method of the eucalyptus leaf extract and its use in the preparation of medicines with inhibitory effect on tumor cell growth . Background technique [0002] Oak tree (Querus mongolica Fisch) is a deciduous tree of the family Fagaceae, the genus Quercus. There are species of Quercus mongolica, Quercus liaotungensis, Quercus mongolica, and oak tree. The leaf margins are serrated and rarely entire. The catkins of the male flowers are drooping, and the female flowers are solitary in the involucre. The nut is solitary, the inner wall of the pericarp is glabrous, and the rudimentary ovule is located outside the base of the seed. The trunk is peculiar and vigorous, the shape of the tree is beautiful and varied, the branches and leaves are luxuriant, it is resistant to pruning, and it is easy to shape. ...

Claims

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Application Information

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IPC IPC(8): A61K36/185A23L1/29A61P35/00A23L33/00
Inventor 王林美岳冬梅夏兴宏李树英
Owner 辽宁省农业科学院大连生物技术研究所
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