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Use of miR-10b gene in stomach cancer diagnosis

A technology of mir-10b, gastric cancer, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc.

Inactive Publication Date: 2015-07-08
HUZHOU CENT HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Clinically used biomacromolecular tumor markers such as carcinoembryonic antigen (CEA) and glycoprotein antigens (CA19-9, CA125) can improve the detection rate of malignant tumors in vivo, especially gastrointestinal malignant tumors, but these markers Can not achieve early diagnosis of gastric cancer with high sensitivity and high specificity

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  • Use of miR-10b gene in stomach cancer diagnosis
  • Use of miR-10b gene in stomach cancer diagnosis
  • Use of miR-10b gene in stomach cancer diagnosis

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Embodiment Construction

[0026] The present invention is further described below in conjunction with embodiment.

[0027] A. The expression level of miR-10b in gastric cancer and the invasion and metastasis of gastric cancer cells.

[0028] a) Detection of expression levels of miR-10b in different gastric cancer cells and normal gastric mucosal epithelial cells

[0029] At the same time, gastric cancer cell lines BGC-803, MGC-803, AGS, BGC-823, MKN-45, SGC-7901, HGC-27 and normal gastric epithelial cell line GES-1 with different differentiation and metastasis abilities were recovered and cultured. When the cells were in the logarithmic growth phase, total RNA was extracted, and the expression of miR-10b in the cells was detected by real-time fluorescent quantitative PCR method. Such as figure 1 As shown, there are large differences in the expression levels of miR-10b in different cell lines. MiR-10B expression in gastric cancer cells compared with GES-1 cells in BGC-803(8.6-fold), MGC-803(11.8-fold...

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Abstract

The invention discloses a use of a miR-10b gene in stomach cancer diagnosis. Over-expressed miR-10b can promote growth, invasion and transfer of stomach cancer cells in vivo and in vitro. Expression of miR-10b in stomach cancer tissue is closely related to tumor size, tumor primary position, tumor intrusion depth and tumor grade malignancy of patients with stomach cancer. Blood plasma miR-10b detection can be used for stomach cancer diagnosis and combined use of the blood plasma miR-10b detection and CA742 detection can be used for diagnosis of cancers. miRNA provides a certain application value for stomach cancer diagnosis and provides favorable technical support for stomach cancer diagnosis and prognosis.

Description

technical field [0001] The invention relates to the role of a miR-10b gene in cancer, in particular to the application in the diagnosis of gastric cancer. Background technique [0002] The ability of invasion and metastasis of malignant tumors is a major difficulty in tumor treatment. In recent years, the emergence of microRNA (microRNA, miRNA) has provided new ideas and approaches for studying the mechanism of tumor occurrence, invasion and metastasis. MicroRNA (miRNA) is a kind of endogenous, conserved and stable non-coding short single Stranded RNA regulates the expression of target genes at the post-transcriptional level. miRNA plays an important role in physiological and pathological processes such as body development, cell proliferation, apoptosis, and tumor development, and has become a hot spot in current life science research. miRNA (microRNA) is a 19-25nt non-coding single-stranded small molecule RNA widely present in organisms, which degrades target mRNA or inhib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 姚行马志红戴利成
Owner HUZHOU CENT HOSPITAL