Carrier for gene introduction use, gene introduction agent, methods for producing said carrier and said gene introduction agent, and method for introducing gene into cell

Abstract

The present invention provides; a novel gene introduction method which enables a gene to be introduced more safely and more freely, particularly a method for introducing a gene into a specified site in the brain safely and freely; a carrier for gene introduction use, which comprises a nano-particle and a substance capable of binding to a vector for gene introduction and has functional groups involved in the induction of phagocytosis by cells, wherein the substance capable of binding to a vector for gene introduction can bind to the surface of the nano-particle through some of the functional groups and another some of the functional groups remain unbound to the substance capable of binding to a vector for gene introduction ; and a gene introduction agent, in which a vector for gene introduction is bound to the substance capable of binding to a vector for gene introduction in the carrier for gene introduction.

Description

technical field [0001] The present invention relates to a gene transfer agent, a gene transfer method using the gene transfer agent, and a method for producing the gene transfer agent. The gene transfer agent is characterized in that heparin or heparan sulfate is bound to the surface, Contains a vector for gene transfer. Background technique [0002] So far, gene transfer methods have been developed for expressing the gene in target cells, tissues, organs, etc., using a virus into which a gene of interest has been recombinantly incorporated. For example, a method of injecting a gene-recombined virus into a blood vessel and moving it to a target organ through the bloodstream, and a method of directly injecting it into a target organ using a syringe or the like have been developed. However, in such a method, there are problems in that the injected virus spreads, the virus concentration decreases at the desired site and a sufficient gene expression level cannot be obtained, an...

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Problems solved by technology

However, in such a method, there are problems in that the injected virus spreads, the virus concentration decreases at the desired site and a sufficient level of gene expression cannot be obtained, and the gene is expressed at an unintended site, which may cause adverse effects.

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