Method for remediating halogenated hydrocarbon-contaminated sites by using reductive dechlorinase

Abstract

The invention relates to a contaminated site remediation method which is mainly used for remedying a contaminated site mainly containing halohydrocarbon pollutants, in particular to remediation adopting the reductive dechlorination enzyme. According to the specific efficiency of the reductive dechlorination enzyme on the halohydrocarbon pollutants, one method for improving the activity of the reductive dechlorination enzyme is proposed, so that the halohydrocarbon DNAPL (dense non-aqueous phase liquid) pollutants in the deep position of the contaminated site are rapidly and thoroughly removed. The method for remedying the halohydrocarbon contaminated site through the reductive dechlorination enzyme comprises the step that biological enzyme is injected to the underground part of the halohydrocarbon contaminated site and transferred to a pollution source area for pollutant degradation, and is characterized in that the biological enzyme is the reductive dechlorination enzyme, and the reductive dechlorination enzyme is prepared to form a liquid enzyme preparation containing the reductive dechlorination enzyme, diethyl aminoethyl synthetic plasma and polymeric alcohol. The activity of the enzyme in the reductive dechlorination enzyme preparation prepared with the method can keep longer than one week, and the practicability of the preparation for site remediation is guaranteed.

Description

technical field [0001] The invention relates to a method for remediating polluted sites, which is mainly used for the remediation of polluted sites mainly containing halogenated hydrocarbon pollutants, and in particular relates to the remediation using reductive dechlorination enzymes. Background technique [0002] As a common chemical raw material, halogenated hydrocarbons are widely used as organic solvents in industries such as dry cleaning, mechanical processing, and electronic processing. Large quantities of halocarbons leak from buried storage tanks or are released into the environment as waste after use. Halogenated hydrocarbons have three effects of carcinogenicity, teratogenicity and mutagenicity on the human body, and are extremely toxic. Therefore, once released into the environment, they will bring great risks to human health and environmental ecological safety. Halocarbons are denser than water and relatively less soluble. Therefore, when they are discharged in...

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Problems solved by technology

However, the laccase used in this method is an oxidase, and DDT is difficult to be completely oxidized. At the same time, the activity of the biological enzyme is relatively short, which affects the effect of using it for remediation of contaminated sites.

In addition, this method is limited to the use of laccase for remediation of contaminated soil, and it is difficult to produce remediation effects on halocarbon pollutants that are easily deposited deep into the contaminated site or into the aquifer