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Preparation with hypothyroidism resisting effect, and preparation method thereof

A preparation and action technology, applied in the processing and application fields of aquatic product fermentation products, can solve the problems of insignificant curative effect, inability to reduce patients' anti-thyroid autoantibodies, ineffective immune abnormalities, etc., and achieve the effect of easy absorption

Inactive Publication Date: 2015-08-12
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current clinical hypothyroid drugs have no effect on immune abnormalities, and cannot reduce the high titer of anti-thyroid autoantibodies in patients' serum
Although it has been reported abroad that long-term use of thyroid hormones has a certain inhibitory effect on the production of autoimmune antibodies, the mechanism of action is to supplement the body's thyroid hormones exogenously, so that the hypothyroid function can be restored, and long-term use can significantly increase High thyroid hormone levels in patients, and through the hypothalamus-pituitary system, inhibit the release of thyroid-stimulating hormone TSH from the pituitary gland, which is not conducive to the recovery of normal thyroid function in patients, and the clinical symptoms of hypothyroid patients with normal or borderline thyroid function The curative effect is not obvious

Method used

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  • Preparation with hypothyroidism resisting effect, and preparation method thereof
  • Preparation with hypothyroidism resisting effect, and preparation method thereof
  • Preparation with hypothyroidism resisting effect, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. After the fresh scallops are ground into a homogenate with a pulverizer, add 10 volumes (w / v) of distilled water of the scallop homogenate to prepare a homogenate, and then add 1 wt% glucose (v / w) of the homogenate volume to the homogenate. ) and 1 wt% sodium glutamate (v / w) of the homogenate volume to prepare a culture solution rich in sodium glutamate; the culture solution was sterilized at 121° C. for 15 minutes. After cooling, Enterococcus avium was inoculated with 1% inoculum amount (v / v), and fermented and cultivated at 37° C. for 4 days to obtain a scallop fermentation broth with a GABA content of 3.7 g / L.

[0034] The Enterococcus avium (Enterococcus avium) was preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee. The preservation date was May 20, 2014. The preservation address was No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the number was CGMCC NO.9184.

[0035] 2. Separation ...

Embodiment 2

[0040] 1. Anchovy boiled waste liquid, add 5 times the volume of waste liquid (v / v) distilled water to make a solution, add 3wt% glucose (v / w) of the solution volume and 2wt% sodium glutamate of the solution volume to the solution (v / w), formulated as a culture solution rich in sodium glutamate. The culture solution was sterilized at 121°C for 20 minutes. After cooling, Enterococcus avium was inoculated with 1% inoculum amount (v / v), and fermented and cultivated at 37° C. for 4 days to obtain an anchovy waste liquid fermentation broth with a GABA content of 5.1 g / L.

[0041] The Enterococcus avium (Enterococcus avium) is preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the number is CGMCC No. 9184.

[0042] 2. Separation and purification of GABA in the fermentation broth of anchovy waste liquid rich in GABA.

[004...

Embodiment 3

[0047] 1. After the fresh krill is ground into a homogenous slurry with a pulverizer,

[0048] Add 5 volumes (w / v) of distilled water of the krill homogenate quality to prepare a homogenate, then add 1 wt% glucose (v / w) of the homogenate volume and 1 wt% sodium glutamate of the homogenate volume to the homogenate (v / w), formulated as a culture solution rich in sodium glutamate; the culture solution was sterilized at 121°C for 15 minutes. After cooling, Lactobacillus brevis was inoculated, and fermented and cultured at 37° C. for 3 days to obtain a krill fermentation broth with a GABA content of 3.4 g / L.

[0049] The Lactobacillus brevis was purchased from China General Microorganism Culture Collection Center, Beijing, with the number CGMCC 1.2028.

[0050] 2. Separation and purification of GABA in the GABA-rich krill fermentation broth.

[0051] 1) Detoxification of GABA-rich krill fermentation broth: centrifuge at 5000r / min for 10 minutes, take the upper liquid and store it...

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Abstract

The invention belongs to the field of technologic processing and application of aquatic product fermentation products, and concretely relates to a preparation method of an aquatic product-derived GABA with a hypothyroidism resisting effect. The method comprises the following steps: grinding a raw material to form a homogenate, adding water, adding glucose and monosodium glutamate to obtain a monosodium glutamate-containing culture solution; inoculating microbes into the culture solution, and carrying out fermentation culturing at 35-40DEG C for 2-14d to obtain a GABA-rich fermentation solution; removing thalli from the GABA-rich fermentation solution, decoloring, allowing the obtained solution to go through cationic resin, and collecting a GABA-rich eluate through a collector; and carrying out reduced pressure concentration on the eluate, and carrying out ethanol drying crystallization to obtain separated and purified GABA. The separated and purified GABA is processed to prepare an aqueous solution, and the aqueous solution is used to feed hypothyroidism model mice, and detection of thyroid function related various indexes is carried out to determine the hypothyroidism resisting effect of the GABA. The hypothyroidism resisting GABA has the characteristics of substantial curative effect, safety, convenience and no toxic side effects.

Description

technical field [0001] The invention belongs to the field of processing and application of aquatic product fermentation products, and in particular relates to a preparation with anti-hypothyroidism effect and a preparation method thereof. Background technique [0002] At present, the total amount of aquatic product farming in my country accounts for 60% of the world, and the resources are extremely rich. Aquatic products are rich in nutrition, delicious in taste, and have many health functions, and have important food value and economic value. With the development of modern separation technology and biotechnology, people's use of aquatic products is no longer limited to mariculture, and its research involves many fields such as ecology, aquaculture, medicine, toxicology, genetics and molecular biology. The high-value development and utilization of aquatic product resources will provide humans with ideal marine food and marine medicines, and create macroeconomic benefits, wh...

Claims

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Application Information

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IPC IPC(8): A61K31/197A61P5/14C12P13/00
Inventor 李鹏程杨皓月邢荣娥刘松于华华秦玉坤李克成陈晓琳李荣锋
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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