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Up-converting NaYF4:Yb<3+>,Tm<3+>nanocrystal-hypocrellin complex preparation method and applications of the prepared complex in photosensitive therapy

A technology of oleocanthal and nanocrystals, which is applied in the field of photosensitizers, can solve the problems of weak tissue penetrating ability of light source, inability to achieve tumor treatment, etc., and achieves the effects of low dark toxicity, long excitation wavelength and high phototoxicity

Inactive Publication Date: 2015-08-12
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Taking advantage of this property, the development of upconverting NaYF 4 : Yb 3+ , Tm 3+ The nanocrystal-hypocretin complex can overcome the difficulty that hypocrellin excitation light source has weak tissue penetration ability and cannot realize the treatment of solid tumors in vivo

Method used

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  • Up-converting NaYF4:Yb&lt;3+&gt;,Tm&lt;3+&gt;nanocrystal-hypocrellin complex preparation method and applications of the prepared complex in photosensitive therapy
  • Up-converting NaYF4:Yb&lt;3+&gt;,Tm&lt;3+&gt;nanocrystal-hypocrellin complex preparation method and applications of the prepared complex in photosensitive therapy
  • Up-converting NaYF4:Yb&lt;3+&gt;,Tm&lt;3+&gt;nanocrystal-hypocrellin complex preparation method and applications of the prepared complex in photosensitive therapy

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Take a 50mL three-necked flask, add 1mmol of Ln(CF 3 COO) 3 (Y:Yb:Tm=78%:20%:2%), 10mmol sodium fluoride (NaF) and 20ml organic solvent (3ml oleic acid / 17ml octadecene), heated to 160 ° C, in anhydrous and oxygen-free atmosphere Continue heating in medium for 1h. Then the temperature was raised to 320° C. and maintained for 1 h while magnetic stirring, and naturally cooled to room temperature after the reaction was completed. Add absolute ethanol to the obtained product, obtain a precipitate after centrifugation, and repeatedly wash with water and ethanol three times, then wash the product in hydrochloric acid (pH=4.0), and extract with a mixed solvent of water / ether to obtain a product without oleic acid on the surface Modified, pure, upconverting NaYF that can be stably dispersed in aqueous phase 4 : Yb 3+ , Tm 3+ nanocrystals. Then, add 200uL HA solution (15mM, DMSO to help dissolve) into the system, stir magnetically at room temperature for 24h, and use twice ...

Embodiment 2

[0039] Embodiment 2 is basically the same as Example 1, but the hypocrellin A is replaced by hypocrellin B. upconverting NaYF 4 : Yb 3+ , Tm 3+ The nanocrystal-hypocretin complex was characterized by the same method as in Example 1, and the results were basically similar.

Embodiment 3

[0040] Example 3 is basically the same as Example 1, and the mass ratio of the nanocrystals to hypocretin is 1:0.1˜1:10.

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Abstract

The invention relates to an up-converting NaYF4:yb<3+>,Tm<3+>nanocrystal-hypocrellin complex preparation method, which comprises: synthesizing an up-converting NaYF4:yb<3+>,Tm<3+>nanocrystal with an excitation wavelength of 980 nm and an up-converting fluorescence wavelength of 470 nm by using a hydrothermal method, removing the surface modifier of the nano-crystal under an acid condition to obtain a nanocrystal aqueous solution, adding a hypocrellin solution, incubating, and removing the unadsorbed hypocrellin and the solvent through dialysis so as to obtain the nanocrystal-hypocrellin complex. With the method of the present invention, the hypocrellin is loaded on the up-converting nanocrystal surface to form the complex, such that the hypocrellin long wave sensitizing purpose is achieved, and the disadvantages of short excitation wavelength and shallow therapy light source tissue penetration depth of the hypocrellin are overcome so as to promote the application of the hypocrellin in treatment of solid tumors in vivo. The present invention further relates to applications of the complex prepared through the method in preparation of photosensitive therapy drugs.

Description

technical field [0001] The invention belongs to the technical field of photosensitizers with photodynamic activity, and relates to a long-wave sensitized up-conversion NaYF 4 : Yb 3+ , Tm 3+ Preparation method of nanocrystal-hypocretin complex and application of the prepared complex in photosensitization therapy. Background technique [0002] Photodynamic therapy (PDT), also known as photoradiation therapy or photochemotherapy, is a new type of tumor treatment. The principle is to use the light source to sensitize the phototherapeutic anticancer drug at the tumor site to undergo a photochemical reaction to generate reactive oxygen species to induce tumor damage. In the process of photodynamic therapy to treat tumors, the key factor is to select phototherapeutic drugs with excellent reactive oxygen generation ability. So far, the antitumor activity of various phototherapeutic drugs such as hematoporphyrin derivatives, metallophthalocyanines, chlorin, 5-aminolevulinic acid...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61P35/00
Inventor 周林周家宏魏少华袁秀雪肖梦思
Owner NANJING NORMAL UNIVERSITY
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