Isoflavone compound contained in tobacco and preparation method and application thereof

A kind of technology of isoflavone and compound, application in anti-oxidation and anti-HIV-1, isoflavone compound and preparation field thereof

Active Publication Date: 2015-08-12
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention isolates a new isoflavone compound with anti-oxidati

Method used

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  • Isoflavone compound contained in tobacco and preparation method and application thereof
  • Isoflavone compound contained in tobacco and preparation method and application thereof
  • Isoflavone compound contained in tobacco and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] - Compound preparation

[0028] Tobacco samples were collected in Yuxi, Yunnan, and the variety was Yunyan-85. Sampling 2.2kg of tobacco samples and pulverizing them to 30 meshes, ultrasonically extracting 3 times with 70% acetone, combining the extracts, filtering, concentrating the extracts under reduced pressure to 1 / 6 of the original volume of the extracts, and filtering out the precipitate after standing , The filtrate was extracted 3 times with ethyl acetate, the ethyl acetate extracts were combined and concentrated into an extract, and 42.8g of extract was obtained.

[0029] After dissolving the extract with an appropriate amount of chloroform, mix the sample with 80g of crude silica gel (80-100 mesh), and pack 1.5kg of silica gel (160-200 mesh) into a column for silica gel column chromatography. The volume ratio of chloroform: acetone (1:0→0: 1) Gradient elution, TLC monitoring and merging the same parts to obtain 8 parts (pure chloroform, chloroform-acetone 20...

Embodiment 2

[0031] - Compound preparation

[0032] Tobacco samples were collected in Yuxi, Yunnan, and the variety was Yunyan-85. Sampling 2.2kg of tobacco sample and pulverizing it to 30 meshes, ultrasonically extracting 3 times with 70% acetone, combining the extracts, filtering, concentrating the extracts under reduced pressure to 1 / 4 of the original volume of the extracts, and filtering out the precipitate after standing , The filtrate was extracted 3 times with ethyl acetate, the ethyl acetate extracts were combined and concentrated into an extract, and 42.7g of extract was obtained.

[0033] After dissolving the extract with an appropriate amount of chloroform, mix the sample with 80g of crude silica gel (80-100 mesh), and pack 1.5kg of silica gel (160-200 mesh) into a column for silica gel column chromatography. The volume ratio of chloroform: acetone (1:0→0: 1) Gradient elution, TLC monitoring and merging the same parts to obtain 8 parts (pure chloroform, chloroform-acetone 20:1,...

Embodiment 3

[0035] - Compound preparation

[0036] Tobacco samples were collected in Yuxi, Yunnan, and the variety was Yunyan-85. Sampling 2.2kg of tobacco samples and pulverizing them to 30 meshes, ultrasonically extracting 3 times with 70% acetone, combining the extracts, filtering, concentrating the extracts under reduced pressure to 1 / 5 of the original volume of the extracts, and filtering out the precipitate after standing , The filtrate was extracted 3 times with ethyl acetate, the ethyl acetate extracts were combined and concentrated into an extract, and 42.9 g of extract was obtained.

[0037] After dissolving the extract with an appropriate amount of chloroform, mix the sample with 80g of crude silica gel (80-100 mesh), and pack 1.5kg of silica gel (160-200 mesh) into a column for silica gel column chromatography. The volume ratio of chloroform: acetone (1:0→0: 1) Gradient elution, TLC monitoring and merging the same parts to obtain 8 parts (pure chloroform, chloroform-acetone 2...

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Abstract

The present invention discloses an isoflavone compound shown as formula (I) contained in tobacco and a preparation method and application thereof. The method is as below: crushing a tobacco sample, conducting ultrasound extraction with 70% acetone 3 times, merging the extracts, filtering, conducting reduced pressure concentration on the filtrate to1/4-1/6 volume of the extract, standing, filtering the precipitate, and extracting with ethyl acetate ester three times to obtain an ethyl acetate extract liquid; and concentrating the extract into an extract, primarily separating the extract by silica gel column chromatography, then further separating the extract by high performance liquid preparative chromatography, so as to obtain the desired isoflavone compound. Antioxidant activity and anti-HIV-1 activity screening is conducted on the compound, and the experimental results show that the compound has strong antioxidant and anti-HIV-1 activity.

Description

technical field [0001] The invention belongs to the field of tobacco chemistry. More specifically, the invention relates to a new isoflavone compound contained in tobacco and a preparation method thereof, and also relates to its application in anti-oxidation and anti-HIV-1. Background technique [0002] Tobacco is one of the most chemical substances among the various plants known to mankind. After decades of research, more than 3,000 individual chemical substances have been identified from tobacco, and many components have not yet been identified. come out. In addition to being mainly used for cigarette production, tobacco can also extract a variety of valuable chemical components; therefore, in addition to being consumed as cigarettes, it is of great significance to strengthen the comprehensive utilization of tobacco and its waste. [0003] Flavonoids widely exist in plants in nature and belong to plant secondary metabolites. According to their structural characteristics,...

Claims

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Application Information

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IPC IPC(8): C07D311/36C07D311/40A61P39/06A61P31/18
CPCC07D311/36C07D311/40
Inventor 申钦鹏刘春波杨光宇张涛张凤梅何沛司晓喜苏钟璧刘志华缪明明
Owner CHINA TOBACCO YUNNAN IND
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