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The Application of Cefnixime Sodium in Expansion of Central Memory T Cells in Vitro

A technology of cefonicid sodium and in vitro expansion, which is applied in the application field of cefonicid sodium in the in vitro expansion of central memory T cells, can solve the problems of influence of treatment effect, short survival time, long duration, etc. Good technical support, safe and effective method, low cost effect

Active Publication Date: 2017-11-07
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, such tumor hyperresponsive cells need to be expanded to clinically required 10 9 -10 11 The number of treatment doses and the duration of the process are relatively long, and time is also a factor that needs to be considered more for cancer patients
Moreover, after a long time of in vitro culture, the cells are prone to aging and their vitality decreases. Most of them are terminally differentiated cells, which have better effects in vitro, but the survival time in vivo is shorter, and the therapeutic effect is affected.

Method used

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  • The Application of Cefnixime Sodium in Expansion of Central Memory T Cells in Vitro
  • The Application of Cefnixime Sodium in Expansion of Central Memory T Cells in Vitro
  • The Application of Cefnixime Sodium in Expansion of Central Memory T Cells in Vitro

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Experimental program
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Embodiment 1

[0020] 1. Preparation of test materials

[0021] Human peripheral blood was provided by Guangzhou Blood Center, and 24 blood samples from normal people were randomly selected in the experiment.

[0022] Main reagents: Fetal bovine serum was purchased from GIBCO Company; RPMI1640 medium was purchased from INVERTROGEN Company; cefnixime sodium was purchased from INVERTROGEN Company; antibodies anti-CD45RA-Texas Red, anti-CCR7-AF700\ anti-CD62L-PE-cy7 was purchased from BD Company; lymphocyte separation medium was purchased from Tianjin Haoyang Biological Products Co., Ltd.; CD4 + T cell negative selection magnetic beads were purchased from BD Company.

[0023] Main experimental instruments: desktop high-speed centrifuge (Eppendorf Centrifuge 5810R), flow cytometer (Beckman Coulter), CO 2 Cell culture incubator (Thermo SCIENTIFIC), biological safety cabinet (ThermoSCIENTIFIC), inverted biological microscope (Leica), magnetic bead sorting frame (BD Pharmigen).

[0024] , cell ...

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Abstract

The present invention provides the application of cefnixime sodium on the expansion of central memory T cells in vitro, the concentration of the cefnixime sodium is 5-40uM, the optimum concentration is 20uM, and the central memory T cells are CD4+ T cells. The present invention discloses for the first time the application of cefnixime sodium in in vitro T cell culture. The method is safe, effective and low in cost, and provides good technical support for the extensive development of adoptive immunotherapy.

Description

technical field [0001] The invention belongs to the technical field of in vitro expansion of central memory T cells, and more specifically relates to the application of cefnixime sodium in in vitro expansion of central memory T cells. Background technique [0002] Cefnicest sodium (English: CEFONICID SODIUM) is commonly used clinically for lower respiratory tract infection, urinary tract infection, sepsis, skin and soft tissue infection, bone and joint infection caused by sensitive bacteria, and can also be used for surgery to prevent infection. A single dose of 1 g cefnixime before surgery can reduce the incidence of postoperative infection due to contamination or potential contamination during surgery. The use of cefanixime during caesarean section (after the umbilical cord is cut) can reduce the incidence of some postoperative infections. [0003] Since T cells play an important role in the immune system, more and more research groups are trying to use T cell adoptive th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0783
Inventor 张辉张译文
Owner SUN YAT SEN UNIV
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