Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of ampicillin in in-vitro amplification of central memory T cells

A technology of ampicillin and in vitro amplification, applied in the application field of ampicillin in the in vitro expansion of central memory T cells, can solve the problems of treatment effect impact, short survival time, long duration, etc., and achieve good technical support, Low cost, safe and effective method

Active Publication Date: 2015-08-12
SUN YAT SEN UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such tumor hyperresponsive cells need to be expanded to clinically required 10 9 -10 11 The number of treatment doses and the duration of the process are relatively long, and time is also a factor that needs to be considered more for cancer patients
Moreover, after a long time of in vitro culture, the cells are prone to aging and their vitality decreases. Most of them are terminally differentiated cells, which have better effects in vitro, but the survival time in vivo is shorter, and the therapeutic effect is affected.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of ampicillin in in-vitro amplification of central memory T cells
  • Application of ampicillin in in-vitro amplification of central memory T cells
  • Application of ampicillin in in-vitro amplification of central memory T cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Preparation of test materials

[0021] Human peripheral blood was provided by Guangzhou Blood Center, and 24 blood samples from normal people were randomly selected in the experiment.

[0022] Main reagents: fetal bovine serum was purchased from GIBCO; RPMI1640 medium was purchased from INVERTROGEN; ampicillin was purchased from INVERTROGEN; antibodies anti-CD45RA-Texas Red, anti-CCR7-AF700\ anti- CD62L-PE-cy7 was purchased from BD Company; lymphocyte separation fluid was purchased from Tianjin Haoyang Biological Products Co., Ltd.; CD4 + T cell negative selection magnetic beads were purchased from BD Company.

[0023] Main experimental instruments: desktop high-speed centrifuge (Eppendorf Centrifuge 5810R), flow cytometer (Beckman Coulter), CO 2 Cell culture incubator (Thermo SCIENTIFIC), biological safety cabinet (Thermo SCIENTIFIC), inverted biological microscope (Leica), magnetic bead sorting frame (BD Pharmigen).

[0024] cell culture

[0025] 2.1 Cell extr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an application of ampicillin in the in-vitro amplification of central memory T cells. The concentration of ampicillin is in a range of 5-40 [mu]M, the optimal concentration is 20 [mu]M, and the central memory T cells are CD4+ T cells. The invention discloses the application of ampicillin in the in-vitro T cell culture for the first time, the method is safe and effective, the cost is low, and good technical support is provided for the wide application of adoptive immunotherapy.

Description

technical field [0001] The invention belongs to the technical field of in vitro expansion of central memory T cells, and more specifically relates to the application of ampicillin in in vitro expansion of central memory T cells. Background technique [0002] Ampicillin is a broad-spectrum penicillin antibiotic commonly used in clinical practice. It is a broad-spectrum semi-synthetic penicillin with extremely low toxicity. The antimicrobial spectrum is similar to that of penicillin, and the bacteria that are sensitive to penicillin are less effective, and the antibacterial effect on Streptococcus viridans is similar to or slightly stronger than that of penicillin. Its efficacy against diphtheria bacillus, tetanus bacillus and actinomycetes is basically the same as that of penicillin. The effect on Enterococcus and Listeria is better than that of benzyl penicillin. It has no antibacterial effect on drug-resistant Staphylococcus and other bacteria that can produce penicillina...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783
Inventor 张辉张译文
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com