A method for improving the yield of sea red astaxanthin produced by fermentation
A technology of red shrimp and green pigment, applied in the field of microorganisms, can solve problems such as high prices and failure to meet market demand, and achieve the effects of reducing production costs, reducing fermentation operating costs, and reducing energy consumption
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Embodiment 1
[0027] This example provides a method for improving the yield of sea red astaxanthin produced by fermentation, which is realized by the following steps: using Rhodotorula gelatinum as the fermentation strain, according to the inoculum size of 2%, inoculating the rhodotorula gelatinous yeast into the culture medium , the medium includes: glucose 60g / L, yeast powder 3g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 0.5g / L, urea 1g / L and NaCl0.3g / L, the pH of this culture medium is 6.5, in described culture medium, add calcium carbonate and magnesium sulfate, adopt the fermentor of 5L to carry out fermentation, adjust in fermentation process The ventilation rate and the rotation speed of the fermenter, the fermentation temperature was maintained at 25°C, and the fermentation pH value was 5.5 for 3 days of fermentation.
[0028] The calcium carbonate is added in an amount of 0.1 g / L, and the magnesium sulfate is magnesium sulfate heptahydrate, and the added amount is 0.1-0.7 g / L.
[0029] T...
Embodiment 2
[0032] This example provides a method for improving the yield of sea red astaxanthin produced by fermentation, which is realized by the following steps: using Rhodotorula gelatinum as the fermentation strain, and inoculating the rhodotorula gelatinus into the culture medium according to the inoculum size of 10% In, the medium includes: glucose 130g / L, yeast powder 10g / L, KH 2 PO 4 1.5g / L, K 2 HPO 4 1.5g / L, urea 5g / L and NaCl9g / L, the pH of this culture medium is 7.5, in described culture medium, add calcium carbonate and magnesium sulfate, adopt the fermentor of 5L to carry out fermentation, regulate ventilation rate during fermentation and the rotation speed of the fermenter, the fermentation temperature was maintained at 25°C, and the pH value was 7.5 for 3 days of fermentation.
[0033] The addition amount of described calcium carbonate is 0.5g / L, and described magnesium sulfate is magnesium sulfate heptahydrate, and addition amount is 0.7g / L.
[0034] The ventilation...
Embodiment 3
[0037] This example provides a method for improving the yield of sea red astaxanthin produced by fermentation, which is realized by the following steps: using Rhodotorula gelatinum as the fermentation strain, according to the inoculum size of 6%, inoculating the rhodotorula gelatinous yeast into the culture medium In, the medium includes: glucose 95g / L, yeast powder 7g / L, KH 2 PO4 1g / L, K 2 HPO 4 1g / L, urea 3g / L and NaCl4.65g / L, the pH of this culture medium is 6.5, add calcium carbonate and magnesium sulfate in described culture medium, adopt the fermentation tank of 5L to carry out fermentation, regulate ventilation during fermentation The fermentation temperature was maintained at 31°C, and the fermentation pH value was 6.5 for 3 days.
[0038] The addition of described calcium carbonate is 0.3g / L, and described magnesium sulfate is magnesium sulfate heptahydrate, and addition is 0.4g / L.
[0039] The ventilation rate is 1.65L / min in 1-12h during the fermentation proces...
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