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A kind of Agave bulbil induction method

A technique for inducing agave beads and buds, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of strong public welfare, substantial cost savings, and broad prospects for industrialization development

Inactive Publication Date: 2016-10-05
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report about the method for inducing buds of agave bulbils

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  • A kind of Agave bulbil induction method
  • A kind of Agave bulbil induction method
  • A kind of Agave bulbil induction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for inducing buds of agave bulbils, the steps are as follows:

[0034] 1) Material collection method: select the bolted moss and plump Phnom Penh agave ( Agave americanaL.var . marginata alba The bulbil of Trel) is material;

[0035] 2) Culture medium preparation: prepare bud induction medium, bud proliferation medium, and rooting medium for bulbils respectively. The pH value of the medium is 5.6, and the thickness of the medium is 1.4cm;

[0036] The bud induction medium of the bulbil: MS+2.0mg·L -1 6-BA+0.5mg·L -1 NAA+1.0mg·L -1 KT+0.01mg·L -1 TDZ +7.0g·L -1 Ag+20g L -1 Su+1.0 g·L -1 Ac,

[0037] The bud proliferation medium: MS+1.0mg·L -1 6-BA+0.02mg·L -1 TDZ+0.3mg·L -1 NAA+1.0g·L -1 Peptone +7.0g·L -1 Ag+20g L -1 Su +1.5 g·L -1 Ac,

[0038] The rooting medium: 1 / 2MS+0.1mg·L -1 NAA+0.5mg·L -1 IBA+ 7.0g·L -1 Ag+20g L -1 Su+1.5 g·L -1 Ac;

[0039] 3) Disinfection treatment of materials: Rinse the surface of the bulbils cut from the p...

Embodiment 2

[0046] A method for inducing buds of agave bulbils, the steps are as follows:

[0047] 1) Material collection method: select the bolted moss and plump Phnom Penh agave ( Agave americanaL.var . marginata alba The bulbil of Trel) is material;

[0048] 2) Culture medium preparation: prepare bud induction medium, bud proliferation medium, and rooting medium for bulbils respectively. The pH value of the medium is 5.7, and the thickness of the medium is 1.5 cm;

[0049] The bud induction medium of the bulbil: MS+2.0mg·L -1 6-BA+0.5mg·L -1 NAA+1.0mg·L -1 KT+0.01mg·L -1 TDZ +7.0g·L -1 Ag+20g L -1 Su+1.0 g·L -1 Ac,

[0050] The bud proliferation medium: MS+1.0mg·L -1 6-BA+0.02mg·L -1 TDZ+0.3mg·L -1 NAA+1.0g·L -1 Peptone +7.0g·L -1 Ag+20g L -1 Su +1.5 g·L -1 Ac,

[0051] The rooting medium: 1 / 2MS+0.1mg·L -1 NAA+0.8mg·L -1 IBA+ 7.0g·L -1 Ag+20g L -1 Su+1.5 g·L -1 Ac;

[0052] 3) Disinfection treatment of materials: Rinse the surface of bulbils cut from the plan...

Embodiment 3

[0059] A method for inducing buds of agave bulbils, the steps are as follows:

[0060] 1) Material collection method: select the bolted moss and plump Phnom Penh agave ( Agave americanaL.var . marginata alba The bulbil of Trel) is material;

[0061] 2) Culture medium preparation: prepare bulbil bud induction medium, bud proliferation medium, and rooting medium respectively. The pH value of the medium is 5.8, and the thickness of the medium is 1.6 cm;

[0062] The bud induction medium of the bulbil: MS+2.0mg·L -1 6-BA+0.5mg·L -1 NAA+1.0mg·L -1 KT+0.01mg·L -1 TDZ +7.0g·L -1 Ag+20g L -1 Su+1.0 g·L -1 Ac,

[0063] The bud proliferation medium: MS+1.0mg·L -1 6-BA+0.02mg·L -1 TDZ+0.3mg·L -1 NAA+1.0g·L -1 Peptone +7.0g·L -1 Ag+20g L -1 Su +1.5 g·L -1 Ac,

[0064] The rooting medium: 1 / 2MS+0.1mg·L -1 NAA+1.0mg·L -1 IBA+ 7.0g·L -1 Ag+20g L -1 Su+1.5 g·L -1 Ac;

[0065] 3) Disinfection treatment of materials: Rinse the surface of bulbils cut from the plant wit...

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Abstract

The invention particularly relates to an inducing method for a maguey bulblet. The method comprises the following steps: taking materials; preparing a culture medium; carrying out disinfection treatment on the materials; carrying out induced cultivation on the bulblet; carrying out enrichment culture; carrying out root induction; and carrying out plant regeneration. The invention aims at providing an inducing method for a maguey bulblet. A technical support is provided for scale management and industrialization. Plump maguey bulblets are selected as the inducing material, and are subjected to induced cultivation in a sterile condition for 20-30 days, so as to germinate strong sprouts; and a lot of proliferation buds can be produced after enrichment culture for 35-50 days, so that the propagation coefficient is high; the proliferation time is short; the growth process is greatly shortened; seedlings can be formed after root induction for 25-35 days; the transplanting survival rate can be up to over 93%; a lot of cost is reduced; the survival rate after the seedlings are transplanted is high; and the seedlings are healthy, forceful, good in growth vigor, regular and consistent.

Description

technical field [0001] The invention specifically relates to a method for inducing agave bulbils. Background technique [0002] Agave americana L., also known as Agave americana L., belongs to the family Agaveaceae. Agave americana is a perennial evergreen large herb with tall plants. The leaves are arranged in rosettes, usually 30-40 pieces, sometimes 50-60 pieces, large, fleshy, oblanceolate linear, 1-2 meters long, 15-20 cm wide in the middle, 10-12 cm wide at the base, leaf margins It has sparse spines, with 1 hard spike at the top, the spines are dark brown, 1.5-2.5 cm long. The panicle is large, 6-12 meters long, multi-branched; the flowers are yellow-green; the perianth tube is about 1.2 cm long, and the perianth lobes are 2.5-3 cm long; the stamens are about twice as long as the perianth. Capsule oblong, about 5 cm long. Because of its strong leaves, evergreen all year round, yellow-green flowers, there are very few bulbils formed on the inflorescences after flowe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 杨志坚何碧珠吴仁烨翁丹婷王和义
Owner FUJIAN AGRI & FORESTRY UNIV
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