Culture medium for culturing mesenchymal stem cells

A mesenchymal stem cell and culture medium technology, applied in the field of culture medium for mesenchymal stem cells, to achieve broad application prospects and clear chemical composition

Inactive Publication Date: 2015-09-09
河南中科干细胞基因工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it can be considered that there may be many uncertain factors in the stability of MSC cultu...

Method used

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  • Culture medium for culturing mesenchymal stem cells
  • Culture medium for culturing mesenchymal stem cells
  • Culture medium for culturing mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A medium for cultivating mesenchymal stem cells, the medium has the following composition including two types of components, a base medium and a medium additive,

[0045] Medium additives include cell growth factor combinations; insulin; trace elements; lipid substances; vitamin substances; hormone substances; protein molecules; amino acid substances; other compounds.

[0046] Formulas for serum-free media

[0047]

[0048]

Embodiment 2

[0050] A culture medium for culturing mesenchymal stem cells, the culture medium comprises two types of components, a basic medium and a medium additive.

[0051] Medium additives include cell growth factor combinations; insulin; trace elements; lipid substances; vitamin substances; hormone substances; protein molecules; amino acid substances; other compounds.

[0052] Formulas for serum-free media

[0053]

[0054]

Embodiment 3

[0056] A culture medium for culturing mesenchymal stem cells, the culture medium comprises two types of components, a basic medium and a medium additive.

[0057] Medium additives include cell growth factor combinations; insulin; trace elements; lipid substances; vitamin substances; hormone substances; protein molecules; amino acid substances; other compounds.

[0058] Formulas for serum-free media

[0059]

[0060]

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Abstract

The invention relates to the field of cell and tissue culture, in particular to a culture medium for culturing mesenchymal stem cells. Aiming at the problems that the mesenchymal stem cells exist in an existing bovine serum culture medium and a human serum culture medium, the invention provides the culture medium for culturing the mesenchymal stem cells. According to the technical scheme, the culture medium for culturing the mesenchymal stem cells provided by the invention is characterized by comprising two kinds of components, namely a basal culture medium and culture medium additives; the additives comprise a cell growth factor combination, insulin, trace elements, lipid materials, vitamin substances, hormone-like substances, protein molecules, amino acid substances and other compounds. According to the culture medium for culturing the mesenchymal stem cells, provided by the invention, the zoonosis and the risk of human immune responses in the cell therapy process can be avoided, and the possibility that the natures of the cultured mesenchymal stem cells are different due to the difference among culture system batches can be avoided; the culture medium is suitable for culturing all the mesenchymal stem cells and is broad in prospect.

Description

technical field [0001] The invention relates to the field of cell and tissue culture, in particular to a culture medium for culturing mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells, the English name is mesenchymal stem cells (MSC), refers to a single or a group of cells that meet the international unified standards (Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R , Keating A, Prockop Dj, Horwitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy, 2006; 8(4): 315-7). [0003] In the bone marrow, MSCs belong to non-hematopoietic stem cells, have the ability to differentiate into osteoblasts, chondrocytes, and adipocytes, and have immune regulation and hematopoietic support functions. Recent studies have shown that MSCs are also present in other tissues, including fat, umbilical cord, placenta and muscle. MSC cul...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
Inventor 郭子宽赵靖王娟李乔丽芦俊萍刘瑾尹珊
Owner 河南中科干细胞基因工程有限公司
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