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Method for evaluating insect development rate by use of aldehyde dehydrogenase gene

A technology of acetaldehyde dehydrogenase and development rate, which is applied in the fields of insect molecular ecology and biotechnology, can solve the problems of large manpower and material resources, time-consuming, and evaluation contents, etc., and achieves easy purchase, simple operation and wide material sources. Effect

Active Publication Date: 2015-09-09
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0005] At present, most evaluations of insect growth and development rates are mainly focused on the evaluation of insect ecology data and indicators. The evaluation involves a lot of content and takes a long time. Generally, 1-2 generations of insect populations are required, which takes a long time and requires a lot of manpower and material resources. The cost is high, therefore, it is necessary to establish a method for quickly predicting and judging the development of insects, and to break through the limitations of the research on effective accumulated temperature

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  • Method for evaluating insect development rate by use of aldehyde dehydrogenase gene
  • Method for evaluating insect development rate by use of aldehyde dehydrogenase gene
  • Method for evaluating insect development rate by use of aldehyde dehydrogenase gene

Examples

Experimental program
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Effect test

Embodiment 1

[0085] Embodiment 1. Design and synthesis of primers for amplifying the acetaldehyde dehydrogenase gene

[0086] According to the acetaldehyde dehydrogenase gene of P. asiatica, the following primers were designed and synthesized for amplifying a partial fragment of the gene:

[0087] Upstream primer ALDH-F: 5'-AAATCCTAACCCTGAAGTTCTC-3' (SEQ ID No.1);

[0088] Downstream primer ALDH-R: 5'-ATCAGCCTTATCACCTTCTTGT-3' (SEQ ID No.2).

[0089] The nucleotide sequence of the acetaldehyde dehydrogenase gene of P. asiatica is shown in SEQ ID No.3, and the amino acid sequence of the acetaldehyde dehydrogenase gene is shown in SEQ ID No.4.

Embodiment 2

[0090] Embodiment 2, detect the growth rate of the Asian carlocust

[0091] 1. Divide Oedaleus decorus asiaticus Bei-Bienko (1941) into the following four groups according to their habitats:

[0092] Group I: Asiatic locusts in the phylloxera group;

[0093] Group II: Asiatic locust in Leymus chinensis;

[0094] Group III: Asiatic locust in Stipa cruzi;

[0095] Group IV: Asiatic locust in Artemisia fragrans;

[0096] There were 20 P. asiaticus in each group mentioned above, until the P. asiaticus died naturally.

[0097] Habitat types of the above-mentioned groups (such as Cryptospermia grassa, Leymus chinensis, Stipa cruzi and Artemisia frigidiosa) are used to investigate plant diversity, height, coverage, density, insect Habitat types classified by diversity, etc.

[0098] 2. Extracting the RNA of each group of Tesiliacus asiaticus in group I, II, III and IV respectively, and reverse transcription to obtain the cDNA of each group of asiaticus asiaticus in group I, II, ...

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Abstract

The invention discloses a method for evaluating the insect development rate by use of the aldehyde dehydrogenase gene, particularly application of a substance for detecting expression quantity of the aldehyde dehydrogenase gene to evaluation on the insect development rate. The substance for detecting expression quantity of the aldehyde dehydrogenase gene can be particularly DNA molecules shown in SEQ ID No.1 and a DNA molecule constituted primer pair shown in SEQ ID No.2. When the primer pair and the method which are provided by the invention are adopted for evaluating the insect development rate, only 2-3 days are required, however, about 30-60 days are required when the traditional biological method is adopted. Relevant materials of the method provided by the invention are wide in source and easy to buy, the operation is simple, time-saving and labor-saving, and the method provided by the invention is suitable for being popularized in and applied to insect commodity inspection and detection.

Description

technical field [0001] The invention belongs to the fields of biotechnology and insect molecular ecology, and relates to a method for evaluating the growth rate of insects by using acetaldehyde dehydrogenase gene. Background technique [0002] The temperature in the thermophilic zone and the growth and development of insects are often used as indicators of growth and development in ecology. The development period refers to the number of days it takes to complete a certain development stage (one generation, one worm stage or one age), and the development rate refers to the development speed completed in one day, which is the reciprocal of the development period, that is, V=1 / N In the formula: V is the development rate and N is the development period. [0003] The relationship between temperature and biological growth and development is more concentratedly reflected in the influence of temperature on the growth rate of plants and temperature-changing animals (especially insec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 秦兴虎张泽华吴慧娟张未仲黄训兵马景川
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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