Application of smith reagent in detection of ghb in liquid samples
A reagent and liquid technology, which is applied in the application field of Smith reagent in the detection of GHB in liquid samples, can solve the problems of unavoidable GBL interference and false positives, and achieve the effects of reliable reaction results, stable properties and high detection sensitivity.
Inactive Publication Date: 2017-10-03
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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Problems solved by technology
[0003] Therefore, how to quickly detect and detect GHB components in liquid test materials and control its illegal abuse requires a fast and accurate detection method. At present, the color reaction principle for GHB is the double bond oxidation after GHB lactonization into GBL. Currently, , GHB is a controlled drug in my country, and GBL is not regulated as a widely used spice and pharmaceutical intermediate. Therefore, it is impossible to avoid the interference of GBL on the test results of GHB, which may cause false positives.
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Embodiment 1
[0033] Embodiment 1, the application of Smith reagent in detecting the GHB composition in the liquid test material:
[0034] 1. Preparation of Smith reagent:
[0035] (a) Modified Schweppes reagent: mix reagent A (2 g of glucose dissolved in 20 ml of water) and reagent B (2.4 g of aniline hydrochloride dissolved in 20 ml of ethanol) and dilute to 80 ml with methanol;
[0036] (b) Bromocresol green and methyl orange are mixed in equal volumes to obtain mixed solution A, wherein, bromocresol green: 0.03g of bromocresol green is dissolved in 100ml of 80% methanol solution and oxidized with 0.1N hydroxide It is obtained by adjusting the pH value to 7.0 with sodium, and methyl orange is obtained by dissolving 0.01g of methyl orange in 100ml of methanol, and adjusting the pH value to 7.0 with 0.1N sodium hydroxide;
[0037] (c) Mix the mixed solution A in the step (b) with the modified Schweppes reagent in the step (a) at a ratio of 3:1 (volume ratio) to obtain the Smith reagent. ...
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Abstract
The invention discloses the application of Smith reagent in detecting GHB in liquid specimen. At the same time, a method for detecting GHB in a liquid test material is also disclosed, comprising the following steps: adding Smith reagent dropwise to the liquid test material to be tested, and if the color of the liquid test material to be tested turns green, it proves that the liquid test material to be tested is GHB. The candidate contains GHB, otherwise, the candidate does not contain or the content is lower than the detection limit. The detection method of the present invention has high detection sensitivity and good specificity, and under low-temperature storage, the property of the Smith reagent remains stable for at least half a year, and the reaction result is reliable.
Description
technical field [0001] The invention belongs to the detection field, and in particular relates to the application of Smith reagent in the detection of GHB in liquid sample materials. Background technique [0002] It has been clinically shown that oral administration of 10 mg of GHB will cause temporary memory loss, and one-time use of 20 g will cause poisoning. Symptoms of poisoning include nausea, vomiting, headache, loss of reflexes, loss of consciousness, coma, and even death. Combining alcohol with alcohol will exacerbate its danger. It may be used in crimes, such as: adding to common alcoholic drinks (vodka, white wine, red wine, beer) or beverages (orange juice, green tea, Sprite, Coke) and giving them to the victim to drink for illegal purposes, and some people GHB is taken for sensory stimulation. [0003] Therefore, how to quickly detect and detect GHB components in liquid test materials and control its illegal abuse requires a fast and accurate detection method. ...
Claims
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IPC IPC(8): G01N21/78
Inventor 王蔚昕黄星
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY