Application of Smith reagent in detecting GHB (Gamma-hydroxybutyrate) in liquid sample
A reagent and liquid technology, applied in the application field of Smith reagent in the detection of GHB in liquid samples, can solve problems such as unavoidable GBL interference and false positives, and achieve the effects of reliable reaction results, stable properties and high detection sensitivity.
Inactive Publication Date: 2015-09-09
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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Problems solved by technology
[0003] Therefore, how to quickly detect and detect GHB components in liquid test materials and control its illegal abuse requires a fast and accurate detection method. At present, the color reaction principle for GHB is the double bond oxidation after GHB lactonization into GBL. Currently, , GHB is a controlled drug in my country, and GBL is not regulated as a widely used spice and pharmaceutical intermediate. Therefore, it is impossible to avoid the interference of GBL on the test results of GHB, which may cause false positives.
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Embodiment 1
[0033] Embodiment 1, the application of Smith reagent in detecting the GHB composition in the liquid test material:
[0034] 1. Preparation of Smith reagent:
[0035] (a) Modified Schweppes reagent: mix reagent A (2 g of glucose dissolved in 20 ml of water) and reagent B (2.4 g of aniline hydrochloride dissolved in 20 ml of ethanol) and dilute to 80 ml with methanol;
[0036] (b) Bromocresol green and methyl orange are mixed in equal volumes to obtain mixed solution A, wherein, bromocresol green: 0.03g of bromocresol green is dissolved in 100ml of 80% methanol solution and oxidized with 0.1N hydroxide It is obtained by adjusting the pH value to 7.0 with sodium, and methyl orange is obtained by dissolving 0.01g of methyl orange in 100ml of methanol, and adjusting the pH value to 7.0 with 0.1N sodium hydroxide;
[0037] (c) Mix the mixed solution A in the step (b) with the modified Schweppes reagent in the step (a) at a ratio of 3:1 (volume ratio) to obtain the Smith reagent. ...
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The invention discloses application of a Smith reagent in detecting GHB (Gamma-hydroxybutyrate) in a liquid sample. The invention further discloses a method for detecting the GHB in the liquid sample. The method includes: dropwise adding the Smith reagent into the liquid sample to be detected; if the color of the liquid sample to be detected changes to green, determining that the liquid sample to be detected contains GHB, otherwise, determining that the GHB content of the liquid sample is zero or lower than a detection limit. The method has high detection sensitivity and excellent specificity. Stored at low temperature, the Smith reagent can maintain stable properties for at least six months, and the reaction results are reliable.
Description
technical field [0001] The invention belongs to the detection field, and in particular relates to the application of Smith reagent in the detection of GHB in liquid sample materials. Background technique [0002] It has been clinically shown that oral administration of 10 mg of GHB will cause temporary memory loss, and one-time use of 20 g will cause poisoning. Symptoms of poisoning include nausea, vomiting, headache, loss of reflexes, loss of consciousness, coma, and even death. Combining alcohol with alcohol will exacerbate its danger. It may be used in crimes, such as: adding to common alcoholic drinks (vodka, white wine, red wine, beer) or beverages (orange juice, green tea, Sprite, Coke) and giving them to the victim to drink for illegal purposes, and some people GHB is taken for sensory stimulation. [0003] Therefore, how to quickly detect and detect GHB components in liquid test materials and control its illegal abuse requires a fast and accurate detection method. ...
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IPC IPC(8): G01N21/78
Inventor 王蔚昕黄星
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY