A pipette tip for rapid liquid exchange of suspended cells

Abstract

The invention relates to a pipette head for rapid liquid replacing of suspension cells. The pipette head comprises a pipette head body (1), a sucker (2) and a pipette connector (3). The sucker (2) is arranged at one end of the pipette head body (1), and the pipette connector (3) is arranged at the other end of the pipette head body (1). The end face of the sucker (2) is in an arc shape. A filter element (21) is arranged in the sucker (2). The structure with the filter element is adopted, the filter element can prevent the cells from being sucked away in the nutrient solution absorbing process, and the cells are left in a culture vessel. In addition, the hole diameter of the filter element is smaller than the hole diameter of common suspension cells, and the cells are prevented from being filtered out; furthermore, to avoid the cells from blocking filter holes or adhering onto the filter holes, concave-convex small holes are designed in filtering holes. The problem that liquid replacing of the suspension cells is different and slow is solved. According to the pipette head, the liquid replacing time of the suspension cells is shortened, the damage and loss of the cells are reduced, and convenient and fast liquid replacing can be carried out on the suspension cells.

Description

technical field [0001] The invention relates to consumables for suspension cell culture, in particular to a pipette tip for rapid liquid exchange of suspension cells, and belongs to the innovative technology of the pipette tip for rapid liquid exchange of suspension cells. Background technique [0002] So far, there are two methods for changing the medium of suspended cells. The first method is to collect the cells by centrifugation and add fresh medium. The second method: put the culture bottle upright for a period of time, discard the upper medium, and replace it with fresh medium. These two methods have certain disadvantages. The first centrifugation method has certain damages to the cells, especially for some relatively fragile cells. Some cells with a small amount of cells have few sediments after centrifugation, and they are easy to lose when the supernatant is discarded. Cells, and the operation is relatively cumbersome and takes a long time. For some experiments tha...

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Problems solved by technology

These two methods have certain disadvantages. The first centrifugation method has certain damages to the cells, especially for some relatively fragile cells. Some cells with a small amount of cells have few sediments after centrifugation, and they are easy to lose when the supernatant is discarded. Cells, and the operation is relatively cumbersome and takes a long time. For some experiments that require more accurate time, such as cell drug administration experiments, some drug uptake and efflux pharmacokinetic cell models, need to be collected at various time points Accumulated drugs, the requirements for each time point are relatively precise, and the experimental error is large

If the method of cell centrifugation is used to collect and replace the medium, there will be a certain error in time

The second method of changing the medium will lose a certain amount of cells, which will have a greater impact on the slow-growing and more precious cells, and it will take a certain amount of time to allow the cells to settle upright, so it is not applicable to experiments that require a higher time point.

The pipette gun is a common small instrument in the laboratory. It plays an important role in the quantitative measurement of liquid and the rapid removal of waste liquid. If it can be used to change the liquid of suspended cells, it will solve the difficulty of changing the liquid of suspended cells. The problem is that there is no such matching pipette tip for removing suspended cell culture medium in the market at present.

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