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A constant temperature and humidity perfusion device for mesenteric microcirculation observation and its application

A constant temperature and humidity, mesenteric technology, used in veterinary instruments, medical science, animal restraint devices, etc., can solve the problems of difficult placement, large volume, inability to achieve constant temperature and humidity, etc., to achieve easy movement, small size, good quality The effect of the application foreground

Active Publication Date: 2017-06-06
THE FIRST HOSPITAL OF HEBEI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The Chinese patent "Constant temperature perfusion device for observation of rat mesenteric microcirculation" (Patent No. ZL2005201441671) provides a constant temperature perfusion device for observation experiments, but the observation site of the device cannot achieve constant temperature and humidity, and the device is equipped with a push-pull Door and other structures, the overall volume of the device is too large, it is not easy to be placed on the microscope observation platform, and it cannot be tightly and stably connected with the microscope

Method used

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  • A constant temperature and humidity perfusion device for mesenteric microcirculation observation and its application
  • A constant temperature and humidity perfusion device for mesenteric microcirculation observation and its application
  • A constant temperature and humidity perfusion device for mesenteric microcirculation observation and its application

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Experimental program
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Embodiment 1

[0035] Such as Figure 1-3 As shown, Embodiment 1 includes a perfusion box 1, a fixed plate 2 and a temperature control device 3;

[0036] The perfusion box 1 includes an observation area 4, a heating area 5, a cleaning area 6, a fixed area 12 and a temperature control area 18, the fixed area 12 is arranged on one side of the heating area 5, and the cleaning area 6 and the fixed area 12 are arranged on The other side of heating zone 5;

[0037] A temperature-sensitive probe 8 and a microcirculation observation window 7 are arranged in the observation area 4, and the microcirculation observation window 7 is higher than the plane where the observation area 4 is located and light-transmitting, and the microcirculation observation window 7 is connected to the observation area through a connecting column 15. The bottom plate of 4 is fixedly connected, and the connecting column 15 is made of frosted glass material; an electric heating tube 9 is arranged in the heating zone 5, and t...

Embodiment 2

[0046] Such as Figure 4 shown, combined with Figure 1-3The difference between embodiment 2 and embodiment 1 is that the fixed area 12 and heating area 5 of embodiment 1 perfusion box 1 are on the same side of observation area 4, and the fixing area 12 and heating area 5 of embodiment 2 are respectively on observation area 4 the opposite sides; the fixing plate 2 in the embodiment 1 is provided with the fixing slot 14, and the fixing slot 14 is not provided in the embodiment 2.

[0047] Embodiment 2 is applicable when the experimental animal is large in size, and the fixing plate 2 is not easy to be placed between the objective lens of the microscope and the stage of the mirror arm. When in use, the perfusion box 1 is placed on the stage of the microscope, and the fixing groove 13 of the fixing area 12 is inserted into the microscope stage specimen piece holder to match the outer edge of the fixing screw hole, and the microcirculation observation window 7 is aligned with the...

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Abstract

The invention provides a constant temperature and humidity perfusion device for observation of mesentery microcirculation. The constant temperature and humidity perfusion device comprises a perfusion box, a fixing plate and a temperature control device. The invention provides the application of the constant temperature and humidity perfusion device for microscopic observation of animal mesentery microcirculation at the same time. The constant temperature and humidity perfusion device is small in size and constant in temperature and humidity, a mesentery sample can be conveniently maintained clean, an experiment animal can be firmly fixed, and the constant temperature and humidity perfusion device is in close cooperation with a microscope.

Description

technical field [0001] The invention belongs to the field of medical microscopic observation instruments, in particular to a constant-temperature and constant-humidity perfusion device for mesenteric microcirculation observation and an application thereof. Background technique [0002] The microcirculation area is a place where blood and interstitial fluid directly exchange substances, and its blood flow can be observed with the aid of a microscope. The tissue of the mesentery is thin, which is suitable for directly observing the dilation and contraction and blood flow of the blood vessels in the microcirculation area. [0003] The existing constant temperature perfusion device for observation of mesenteric microcirculation is large in size, which matches the microcirculation microscopes currently on the market. Due to its large size, it is difficult to install and cannot be used in ordinary biological microscopes, especially high-end systems such as Brad multiple projection...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61D3/00A61D7/00
CPCA61D3/00A61D7/00
Inventor 张庆富冯建科
Owner THE FIRST HOSPITAL OF HEBEI MEDICAL UNIV
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