Enterobacter hormaechei CL2013 as well as method for preparing hexavalent chromium restoring bactericide

A technology of Enterobacter hallii and hexavalent chromium, which is applied in the field of preparation of hexavalent chromium repair bacteria, and can solve the problems of high acidity, high organic matter content and high energy consumption in press sewage

Inactive Publication Date: 2015-09-23
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, marigold press wastewater has high acidity and high organic matter content, and there are probl

Method used

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  • Enterobacter hormaechei CL2013 as well as method for preparing hexavalent chromium restoring bactericide
  • Enterobacter hormaechei CL2013 as well as method for preparing hexavalent chromium restoring bactericide
  • Enterobacter hormaechei CL2013 as well as method for preparing hexavalent chromium restoring bactericide

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Embodiment 1

[0046] A strain of Enterobacter hormaechei (Enterobacter hormaechei) CL.2013 was deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on January 27, 2015, with the preservation number CGMCC No.10451, address: Beichen, Chaoyang District, Beijing Institute of Microbiology, Chinese Academy of Sciences, No. 1 Yard, West Road.

[0047] The Enterobacter hallii CL.2013 is isolated and screened from the soil of the chromium slag yard. The strain was cultured on LB medium, the cell shape was rod-shaped, and Gram staining was negative; after 3 days of culture, the color of the colony was milky white, and the shape of the colony was round and smooth. After physiological and biochemical identification and 16S rDNA phylogenetic analysis, the new strain was identified and named Enterobacter hormaechei (Enterobacter hormaechei) CL.2013.

Embodiment 2

[0049] Utilize Enterobacter hallii CL.2013 of the present invention and marigold waste water, waste residue to prepare hexavalent chromium repair solid bacterial agent, and its method comprises:

[0050] (1) Prepare strain activation medium Add glucose 1g / L to LB medium, sterilize for 15-30min, and set aside;

[0051] (2) Preparation of activated seeds Insert the Enterobacter hormaechei CL.2013 strain of the present invention stored in a slant test tube into the medium prepared in (1). Min shaking culture for 12-16 hours to obtain activated seeds;

[0052] (3) Preparation of liquid seed culture solution Adjust the pH of the waste water to 6.5-7.5 with aqueous sodium hydroxide solution to extract lutein from marigolds, and sterilize at 115-121°C for 15-30 minutes;

[0053] (4) Preparation of liquid seeds Take the culture medium prepared in (3) and inoculate the activated seeds prepared in (2), the inoculum amount is 8%-10%, shake culture at 30-37℃, 120-200r / min for 24-48 h , ...

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Abstract

The invention relates to enterobacter hormaechei CL2013 separated from chromium slag storage yard soil, the strain of which is preserved on January 27, 2015, and the preservation number is CGMCC NO.10451. The strain is cultured on an LB culture medium, and the cell is in a rod shape and is Gram-negative; the stain is cultured for 3 days, bacteria colony is in milky white color, the shape of the bacteria colony is circular and smooth, and the strain is determined as the enterobacter hormaechei by virtue of physiological and biochemical reaction and molecular biology. The enterobacter hormaechei CL2013 has good effect for reducing the hexavalent chromium. The invention also discloses a method for preparing hexavalent chromium repairing bactericide by utilizing the enterobacter hormaechei CL2013 strain and marigold processing waste liquid and waste slag. The method is simple, low in cost, free from secondary pollution, convenient to use and wide in market prospect.

Description

technical field [0001] The invention relates to the field of microbes and applications, in particular to the preparation of Enterobacter hallii with reducing effect on hexavalent chromium and the fermentation liquid of the enterobacter hallii and the processing of waste liquid and waste residue by using the enterobacter halloi and marigold A method for preparing hexavalent chromium repairing bacteria agent. Background technique [0002] Chromium (Ⅵ) is the second most harmful heavy metal in groundwater, soil and sediment. It ranks 6th in the US EPA priority pollutant list. It is one of the 117 priority pollutants that are extremely harmful to the human body. It has acute, Subacute and chronic toxicity, carcinogenesis, distortion and mutagenesis. Due to the chromate ion (CrO 4 2- ) and sulfate ion (SO 4 2- ) structural similarity, chromate ions can easily enter the biomembrane through the sulfate ion channel, and further react with certain substances in the cytoplasm, an...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 李维宏岳秀萍马小丽
Owner TAIYUAN UNIV OF TECH
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