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Microflora-based ecology health evaluation method

A microbial community and ecological health technology, applied in the direction of microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of whether the value is suitable without objective basis, doubtful reliability, inappropriate, etc., to achieve the significance of environmental ecology Clear, simple and fast results

Active Publication Date: 2015-10-07
NINGBO UNIV
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Problems solved by technology

But its disadvantages are: (1) too many indicators are involved in the evaluation, but in fact many indicators are difficult to obtain, or the cost is too high; (2) the evaluation standard is formulated under the condition that all indicators can be obtained by default, Therefore, when some indicators are missing, it is obviously inappropriate to apply the original evaluation criteria; (3) The assignment of each indicator and the value of the evaluation standard are artificially given, and there is no objective basis for whether the value is appropriate
The existence of these problems makes it difficult to carry out marine ecosystem health assessment business, and its reliability is widely questioned

Method used

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specific Embodiment

[0023] In August 2013, 98 sampling points were set up in the sea area of ​​Ningbo to collect water samples and sediment samples for chemical analysis, and high-throughput sequencing was performed on the samples after pretreatment such as filtration, DNA extraction, and PCR amplification. The relative abundance of each microbial OTU in the sample. When evaluating with data of different classification levels (phylum, class, order, family, genus, species), except that the data are different, its process is completely consistent with the technical solution method of the present invention. Taking the data of the following microbial genera as an example, the water quality index uses PO 4 - As an example, to illustrate the specific process and method:

[0024] (1) sample i 98 in total i =1,2…,98; analyze 10 water quality indicators for all samples ( j =1,2,…,10), where PO 4 - is the sixth water quality index, that is, its value is x i6 , and calculate the equivalent polluti...

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Abstract

The invention discloses a microflora-based ecology health evaluation method. The method comprises the following steps: 1, sampling water and microbes, and analyzing the water quality; 2, calculating the correlation coefficient between the water quality indexes and the relative abundance of the microbes; 3, screening a microbial flora correlated with a specific water quality index and executing an opposite function; 5, establishing a relationship between the total relative abundance of the correlated microbial flora and the specific water quality index; and 5, mapping with one water quality index of all samples an x axis and Sija-Sijb as a y axis, allowing an S-shaped curve to have two corresponding inflection points when the value of Sija-Sijb is 0 and does not increase, and respectively evaluating three sections divided by the two inflection points to be ecologically healthy, sub-healthy and unhealthy. The method has the advantages of simplicity, fastness, accuracy, impersonality, and clear environment ecology significance, and provides an objective criterion for calculation of the marine site environment capacity.

Description

technical field [0001] The invention belongs to the field of marine ecological environment protection, and in particular relates to a method for evaluating marine ecological health based on microbial communities. Background technique [0002] Ecological health refers to the ability of an ecosystem to maintain its natural attributes, maintain biodiversity and key ecological processes, and continue to perform its service functions. The "Guidelines for Assessment of Coastal Marine Ecological Health" issued by the State Oceanic Administration divides coastal marine ecosystems into ecosystem types such as coral reefs, seagrass beds, mangroves, estuaries, and bays. The basic method is: (1) For various ecosystem types, investigate various water environment indicators, habitat indicators, biological indicators, and biological toxicity indicators; (2) Artificially assign values ​​to these indicators according to certain rules; (3) Add up the assignments of all indicators and calcula...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/689
Inventor 张德民朱建林王凯李建峰陈和平
Owner NINGBO UNIV
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