s100a9 recombinant adenovirus, its construction and application
A technology of S100A9, recombinant adenovirus, applied in the application, virus/phage, recombinant DNA technology and other directions, can solve the problem of lack of objective basis for the specific mechanism, and achieve the effect of stable recombinant adenovirus, convenient purchase and simple operation
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[0032] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, so that those skilled in the art can better understand the present invention and implement it, but the examples given are not intended to limit the present invention.
[0033] 1. Construction and identification of recombinant adenovirus vector plasmid pHBAd-MCMV-RFP-S100A9
[0034] 1. Digestion
[0035] attached figure 1 Is the schematic diagram of the pHBAd-MCMV-RFP overexpression vector and the key points therein.
[0036] not I and Nsi I is the insertion site, for pHBAd-MCMV-RFP vector not I and Nsi I double enzyme digestion, the enzyme digestion system is as follows:
[0037] 20μL enzyme digestion system 37℃ for 3h
[0038] 2μL
Carrier (400ng / μL)
1μL
not I
1μL
Nsi I
2μL
10×buffer
14μL
h 2 o
[0039] 2. Gel recovery and purification
[0040] (1) After DNA electr...
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