Serum-free culture medium for efficiently expressing recombinant human thyroid-stimulating hormone in CHO cells and application thereof

A thyroid-stimulating hormone, serum-free culture medium technology, applied in the field of biomedicine, can solve the problems of easy pollution, complicated operation, and downstream purification burden.

Inactive Publication Date: 2015-10-28
SUZHOU KANGJU BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

During the cultivation process, it is necessary to release the cell fluid and insert fresh medium, and it needs to be repeated several times, and the operation is cumbersome, so there is a risk of contamination and a great bu

Method used

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  • Serum-free culture medium for efficiently expressing recombinant human thyroid-stimulating hormone in CHO cells and application thereof
  • Serum-free culture medium for efficiently expressing recombinant human thyroid-stimulating hormone in CHO cells and application thereof
  • Serum-free culture medium for efficiently expressing recombinant human thyroid-stimulating hormone in CHO cells and application thereof

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Embodiment Construction

[0099] The preparation method of the present invention is specifically described below in conjunction with the examples:

[0100] The present invention discloses a serum-free medium for highly expressing recombinant human thyroid-stimulating hormone in CHO cells, a serum-free medium for highly expressing recombinant human thyroid-stimulating hormone in CHO cells, the medium includes Serum basal media, feed media, and media supplements. The basal medium includes inorganic salts, amino acids, vitamins, trace elements, carbohydrates, surfactants and other organic molecules, and the feed medium includes inorganic salts, amino acids, vitamins, trace elements, carbohydrates, surfactants and other organic molecules

[0101] The composition and content of the medium additives are D-galactose 500-5000mg / L, uracil 100-1000mg / L, Mn 2+ 1-20μmol / L.

[0102] Described inorganic salt contains following composition in every liter of described basal medium:

[0103] CaC1 2 58-291 mg; K...

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Abstract

The invention discloses a serum-free culture medium for efficiently expressing recombinant human thyroid-stimulating hormone in CHO cells and application thereof. The method comprises the steps that serum-free culture medium is used for conducting suspension cultivation on CHO cell strains in a bioreactor and efficiently expressing recombinant human thyroid-stimulating hormone in the CHO cells in a feed supplementation and batch cultivation mode. The invention further discloses a serum-free basic culture medium, a feed supplementation culture medium and a culture medium addition agent which are used in the recombinant human thyroid-stimulating hormone industrial production method. The serum-free culture medium has the main advantages that by means of the recombinant human thyroid-stimulating hormone industrial production method, the recombinant human thyroid-stimulating hormone can be efficiently produced, the product quality is good, safety is high, the expression quantity is large, the culture process is stable, the culture method is concise, the culture period is short, and the serum-free culture medium is suitable for mass production.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to an industrial production method of glycosylated recombinant human thyroid hormone, in particular to a non-toxic thyroid-stimulating hormone that can stably increase the expression of recombinant thyrotropin, shorten the production cycle, and maintain high-quality expression products. Serum medium, industrial production method of glycosylation-modified recombinant human thyroid hormone. Background technique [0002] TSH (thyrotropin, thyroid stimulating hormone) is a macromolecular glycoprotein hormone secreted by the pituitary gland to promote the growth and function of the thyroid gland. Human TSH contains 210 amino acids, and the whole molecule is composed of α-chain subunits and β-chain subunits connected non-covalently. The α-chain subunits are TSH and gonadotropins (including follicle stimulating hormone FSH, luteinizing hormone It is shared by LH and HCG and consists of ...

Claims

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Application Information

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IPC IPC(8): C12P21/02C12Q3/00C12R1/91
Inventor 徐云霞唐瑶王征
Owner SUZHOU KANGJU BIOTECHNOLOGY CO LTD
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