A silicon-based array micro-reaction pool digital PCR chip and its preparation method

A micro-reaction and chip technology, applied in the field of PCR, can solve problems such as the influence of thermal cycle time on PCR amplification efficiency, easy crosstalk in chip micropores, and difficulty in liquid entering the chip evenly, so as to ensure normal PCR amplification efficiency and reduce The effect of volatilization of microporous reaction reagents and shortening of thermal cycle time
CN105039153BActive Publication Date: 2016-09-14宁波胤瑞生物医学仪器有限责任公司
5 Cites 4 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
宁波胤瑞生物医学仪器有限责任公司
Publication Date
2016-09-14

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention belongs to the technical field of PCR (polymerase chain reaction), and particularly relates to a digital PCR chip with silicon substrate arrays and micro-reaction pools and a method for manufacturing the digital PCR chip. The digital PCR chip mainly comprises an upper cover and a chip. Micro-pores which are arrayed to form honeycombs are formed in the chip, and the upper cover is fixed onto a chip groove. The method includes particular steps of selecting a silicon chip with a single polished surface; cleaning the silicon chip; coating a layer of uniform photoresist on the polished surface of the silicon chip in a spinning manner; forming circular graphic arrays by means of exposure; etching silicon by the aid of a dry process in masks, namely, the circular graphic arrays to form micro-pore structures; removing the photoresist and scribing the chip to completely manufacture the digital PCR chip.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of PCR, and in particular relates to a silicon-based array micro-reaction pool digital PCR chip and a preparation method thereof. Background technique

[0002] The application of DNA molecular amplification technology has promoted the development of molecular biology technology, and the precise quantification of DNA copy number is one of the important applications of modern molecular biology and medicine. In 1997, James F. Brown, Jonathan E. Silver and Olga V. Kalinina constructed the prototype of digital PCR (digital polymerase chain reaction, dPCR) technology and obtained the US patent (patent number: US6143496 A). Different from traditional PCR technology, digital PCR fully dilutes nucleic acid samples so that the number of sample templates in each reaction chamber is less than or equal to 1. After PCR amplification, the fluorescent signals of each reaction chamber are statistically analyzed to solve Rea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More