Method for ultrasound-assisted two-aqueous-phase extraction of resveratrol

A resveratrol, ultrasonic-assisted technology, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry and other directions, can solve problems such as unfavorable industrialization, achieve rapid phase separation, easy amplification and continuous operation, and organic solvent content. less effect

Inactive Publication Date: 2015-11-11
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for better recovery rates with less volatile or expensive chemicals compared to traditional methods like alcohol solutions at once but also results in faster separations between different phases. Additionally, this method produces an effective liquid containing low amounts of residual impurities such as polysaccharides while reducing sugars in the final product. Overall, it provides technical benefits over existing techniques by improving their effectiveness on producing highly concentrated products suitable for use in various industries.

Problems solved by technology

This patented technical problem addressed in this patent relates to improving the concentration or yield of certain types of naturally occurring molecules called “polymerizable phenols” like resveratolone during various stages of their manufacture processes.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Enzymatic hydrolysis: take 1.0 g of pulverized Polygonum cuspidatum, add 15 mL of pH4.0 citric acid / sodium citrate buffer and 0.01 g of β-glucosidase, and hydrolyze with shaking in a water bath at 60°C for 2 hours, so that the polydatin in it can be completely converted into Veratrol;

[0020] (2) Ultrasonic-assisted aqueous two-phase extraction: add ammonium sulfate and absolute ethanol to the above enzymolysis solution to form an aqueous two-phase system, so that ammonium sulfate accounts for 27% of the system mass fraction, and absolute ethanol accounts for 18% of the system mass fraction. %. Under the conditions of ultrasonic power of 200W and temperature of 20°C, extract for 60min;

[0021] (3) Phase separation: filter the system obtained in step (2) to remove the medicine dregs, transfer it to a separatory funnel, let it stand for 10 minutes, and divide it into upper and lower phases, and separate the upper phase containing resveratrol to obtain resveratrol ...

Embodiment 2

[0024] (1) Enzymatic hydrolysis: Take 1.0 g of pulverized Polygonum cuspidatum, add 30 mL of pH6.0 citric acid / sodium citrate buffer and 0.01 g of β-glucosidase, and hydrolyze with shaking in a water bath at 40°C for 10 hours, so that the polydatin in it can be completely converted into Veratrol;

[0025] (2) Ultrasound-assisted aqueous two-phase extraction: add ammonium sulfate and absolute ethanol to the above enzymolysis solution to form an aqueous two-phase system, so that ammonium sulfate accounts for 23% of the system mass fraction, and absolute ethanol accounts for 20% of the system mass fraction. %. Under the conditions of ultrasonic power of 300W and temperature of 40°C, extract for 30min;

[0026] (3) Phase separation: filter the system obtained in step (2) to remove the medicine dregs and transfer it to a separatory funnel. After standing for 30 minutes, it is divided into upper and lower phases, and the upper phase containing resveratrol is separated to obtain res...

Embodiment 3

[0028] (1) Enzymatic hydrolysis: Take 1.0 g of pulverized Polygonum cuspidatum, add 25 mL of pH4.5 citric acid / sodium citrate buffer and 0.01 g of β-glucosidase, hydrolyze with shaking in a water bath at 50°C for 6 hours, so that polydatin in it can be completely converted into Veratrol;

[0029] (2) Ultrasonic-assisted aqueous two-phase extraction: add ammonium sulfate and absolute ethanol to the above enzymolysis solution to form an aqueous two-phase system, so that ammonium sulfate accounts for 22% of the system mass fraction, and absolute ethanol accounts for 21% of the system mass fraction. %. Under the conditions of ultrasonic power of 250W and temperature of 30°C, extract for 45min;

[0030] (3) Phase separation: filter the system obtained in step (2) to remove the dregs and transfer it to a separatory funnel. After standing for 20 minutes, it is divided into upper and lower phases, and the upper phase containing resveratrol is separated to obtain resveratrol. Crude a...

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Abstract

The invention discloses a method for ultrasound-assisted two-aqueous-phase extraction of resveratrol, and belongs to the technical field of bioconversion technology and the extraction of natural product effective components from plants. The method comprises the following steps: adding beta-glucosidase to ground polygonum cuspidatum powder, enzymatically hydrolyzing and then extracting resveratrol in enzymatic hydrolysate through an ultrasound-assisted two-aqueous-phase system by using an ethanol/ammonium sulfate two-aqueous-phase system as an extracting agent, and reserving upper phase so as to obtain a crude resveratrol product which can directly undergo subsequent purifying operation. The method disclosed by the invention is rapid in phase splitting and high in recovery rate; a plurality of processes are integrated; by virtue of the two-aqueous-phase extraction, the method not only achieves a purpose of purifying, but also concentrates feed liquid and simultaneously separates the feed liquid from polysaccharide in the system; the adopted two-aqueous-phase system is low in toxicity and conducive to recovery; the method is also easy for process scaling-up and continuous operation; and the method can be directly connected to a subsequent purifying process, without any special treatment.

Description

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Claims

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Application Information

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Owner JIANGNAN UNIV
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