Pleurotus eryngii mushroom strain production method

Abstract

The invention relates to a pleurotus eryngii mushroom strain production method and belongs to the field of edible mushroom strain production methods. The adopted technical scheme is that the pleurotus eryngii mushroom strain production method comprises the following steps: putting a test tube stock culture medium and fresh pleurotus eryngii mushrooms into an inoculation box for sterilization for 30min; performing the following operations under aseptic conditions: selecting a strain source of the size of a rice grain on pleurotus eryngii mushroom and feeding the strain source into the test tube stock culture medium; performing culture at a proper culture temperature of pleurotus eryngii mushroom strain after inoculation, selecting a hypha which reaches 40-50mm the earliest, cutting off a 1-2mm hypha tip from a hypha tip end, feeding the hypha tip into a sterilized stock culture medium plate and performing culture to obtain pleurotus eryngii mushroom stock culture, wherein the strain source is selected by breaking apart a mushroom body and a mushroom cap and selecting the strain source from an aseptic part in the middle of the separation position. The pleurotus eryngii mushroom strain production method solves the problems that the pleurotus eryngii mushroom strain production effect is commonly not good at present, the quality and yield of edible mushrooms are not high and an inoculation process is easily contaminated.

Description

technical field [0001] The invention discloses a method for producing Pleurotus eryngii strains, which belongs to the field of methods for producing edible fungus strains. Background technique [0002] At present, Pleurotus eryngii is basically planted with purchased mother species, and few of them make their own mother species. The reason is that the requirements for the production of strains are high, the selection of provenance and the inoculation process are easy to be polluted, and it is difficult to obtain pure high-quality strains, which makes the process of self-made strains complicated and the cost of seed production is higher. Contents of the invention [0003] The invention overcomes the deficiencies of the prior art, and the technical problem to be solved is to provide a simple method for producing Pleurotus eryngii strains. [0004] For solving the problems of the technologies described above, the technical scheme adopted in the present invention is: a kind o...

AI Technical Summary

Problems solved by technology

The reason is that the requirements for the production of strains are high, the selection of provenance and the inoculation process are easy to be polluted, and it is difficult to obtain pure high-quality strains, which makes the process of self-made strains complicated and the cost of seed production is higher.

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