Pleurotus eryngii mushroom strain production method

A technology of Pleurotus eryngii and strains, applied in the direction of fungi, etc., can solve the problems of high cost of seed production, high requirements, and easy pollution in the inoculation process, and achieve the effects of improving quality and quality, strong anti-virus ability, and not easy to pollute

Inactive Publication Date: 2015-11-25
山西蓓鲜农业科技有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

The reason is that the requirements for the production of strains are high, the selection of provenance and the inoculation process are easy to be polluted,

Method used

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  • Pleurotus eryngii mushroom strain production method

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Embodiment Construction

[0009] The present invention will be further described below in conjunction with specific examples.

[0010] A kind of Pleurotus eryngii strain seed production method, according to the following steps:

[0011] Put the test tube stock culture medium and fresh Pleurotus eryngii into the inoculation box for sterilizing for 30 minutes; perform the following operations under sterile conditions: Pick a piece of provenance the size of a grain of rice on the Pleurotus eryngii, and insert the test tube stock Culture medium; after inoculation, culture at the appropriate temperature for Pleurotus eryngii strains, select the mycelium that first reaches 40-50mm, cut the mycelium tip of 1-2mm from the tip of the mycelium and insert it into the sterilized mother medium plate Cultivate on the above, obtain the female species of Pleurotus eryngii;

[0012] The selection position of the provenance is as follows: the mushroom body and the mushroom cap are separated, and the aseptic part in the...

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Abstract

The invention relates to a pleurotus eryngii mushroom strain production method and belongs to the field of edible mushroom strain production methods. The adopted technical scheme is that the pleurotus eryngii mushroom strain production method comprises the following steps: putting a test tube stock culture medium and fresh pleurotus eryngii mushrooms into an inoculation box for sterilization for 30min; performing the following operations under aseptic conditions: selecting a strain source of the size of a rice grain on pleurotus eryngii mushroom and feeding the strain source into the test tube stock culture medium; performing culture at a proper culture temperature of pleurotus eryngii mushroom strain after inoculation, selecting a hypha which reaches 40-50mm the earliest, cutting off a 1-2mm hypha tip from a hypha tip end, feeding the hypha tip into a sterilized stock culture medium plate and performing culture to obtain pleurotus eryngii mushroom stock culture, wherein the strain source is selected by breaking apart a mushroom body and a mushroom cap and selecting the strain source from an aseptic part in the middle of the separation position. The pleurotus eryngii mushroom strain production method solves the problems that the pleurotus eryngii mushroom strain production effect is commonly not good at present, the quality and yield of edible mushrooms are not high and an inoculation process is easily contaminated.

Description

technical field [0001] The invention discloses a method for producing Pleurotus eryngii strains, which belongs to the field of methods for producing edible fungus strains. Background technique [0002] At present, Pleurotus eryngii is basically planted with purchased mother species, and few of them make their own mother species. The reason is that the requirements for the production of strains are high, the selection of provenance and the inoculation process are easy to be polluted, and it is difficult to obtain pure high-quality strains, which makes the process of self-made strains complicated and the cost of seed production is higher. Contents of the invention [0003] The invention overcomes the deficiencies of the prior art, and the technical problem to be solved is to provide a simple method for producing Pleurotus eryngii strains. [0004] For solving the problems of the technologies described above, the technical scheme adopted in the present invention is: a kind o...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 徐忠华
Owner 山西蓓鲜农业科技有限公司
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