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Application of EPS8L3 gene expression detecting reagents to preparing osteoporosis diagnostic products

A technology for osteoporosis and gene expression, applied in disease diagnosis, biological testing, material inspection products, etc., can solve problems such as inability to give reliable judgments, and achieve early diagnosis, faster gene diagnosis, and timely gene diagnosis Effect

Active Publication Date: 2015-12-02
GUAN BOJIAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main limitation of the above method is that it cannot give a reliable judgment in the early stage of osteoporosis

Method used

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  • Application of EPS8L3 gene expression detecting reagents to preparing osteoporosis diagnostic products
  • Application of EPS8L3 gene expression detecting reagents to preparing osteoporosis diagnostic products
  • Application of EPS8L3 gene expression detecting reagents to preparing osteoporosis diagnostic products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Screening of genes differentially expressed in patients with osteoporosis and normal people

[0048] 1. Research object:

[0049] Randomly select patients who are about to undergo marrow joint replacement and measure bone density. Eight patients with osteoporosis and 8 patients with normal bone density (trauma fractures, without osteoporosis) were selected, aged 52-68 years. Questionnaires were used to investigate the subjects' lifestyle and health status.

[0050] Inclusion criteria for patients with osteoporosis: (1) Those who meet the diagnostic criteria for osteoporosis, refer to the "Recommended Diagnostic Criteria for Osteoporosis in China (Second Draft); (2) All patients have given informed consent.

[0051] Exclusion criteria for patients with osteoporosis: those with secondary osteoporosis.

[0052] 2. Total RNA extraction from blood

[0053] Extraction of total RNA from blood using Biotech Blood RNA Extraction Kit

[0054] (1) Take 250 μl (or 0.2...

Embodiment 2

[0079] Embodiment 2QPCR experiment verification of genes differentially expressed in patients with osteoporosis and normal people

[0080] 1. Research object:

[0081] Randomly select patients who are about to undergo marrow joint replacement, and measure bone density. 50 patients with osteoporosis and 40 patients with normal bone density (traumatic fractures, no osteoporosis) were selected, aged 52-68 years. Questionnaires were used to investigate the subjects' lifestyle and health status.

[0082] Inclusion criteria for patients with osteoporosis: (1) Those who meet the diagnostic criteria for osteoporosis, refer to the "Recommended Diagnostic Criteria for Osteoporosis in China (Second Draft); (2) All patients have given informed consent.

[0083] Exclusion criteria for patients with osteoporosis: those with secondary osteoporosis.

[0084] 2. Extraction of total RNA in blood

[0085]2. Total RNA extraction from blood

[0086] Use Biotech Blood RNA Extraction Kit to extr...

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Abstract

The invention discloses a molecular marker for diagnosing osteoporosis. The molecular marker is an EPS8L3 gene. The molecular marker has the advantages that as proved by research, the mRNA (messenger ribonucleic acid) expression level of EPS8L3 genes in the blood of osteoporosis patients is obviously reduced as compared with normal persons; reagent kits for diagnosing the osteoporosis can be prepared according to correlation between the EPS8L3 genes and the osteoporosis and can be clinically widely applied.

Description

technical field [0001] The invention belongs to the field of molecular diagnosis, and relates to a molecular marker for diagnosing osteoporosis, in particular to the application of the molecular marker-EPS8L3 gene in blood in preparing products for diagnosing osteoporosis. Background technique [0002] Osteoporosis, or osteoporosis, is a group of bone diseases caused by a variety of reasons. Bone tissue has normal calcification, and the ratio of calcium salt to matrix is ​​normal. It is a metabolic bone lesion characterized by a decrease in the amount of bone tissue per unit volume. . In most cases of osteoporosis, the loss of bone tissue is mainly due to increased bone resorption. Characterized by bone pain and susceptibility to fractures. [0003] Epidemiological data show that about 8 million people in the United States suffer from osteoporosis, 20 million people suffer from osteopenia, and 1.5 million patients suffer from fractures due to osteoporosis every year. The ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68
CPCC12Q1/6883C12Q2600/158G01N33/6893G01N2333/47G01N2800/108
Inventor 肖枫杨承刚
Owner GUAN BOJIAN BIOTECH CO LTD
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