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Method for preparing electrochemical luminescence sensor made of nano-composites

A nano-composite material and nano-material technology, applied in the field of electrochemiluminescence sensor preparation, can solve the problems of complex data processing, complicated operation process, expensive equipment and the like, and achieve good biocompatibility, large specific surface area, and detection range. wide effect

Active Publication Date: 2015-12-02
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these traditional analysis methods have strong specificity and high sensitivity, they also have some shortcomings, such as: complex operation process, long time-consuming, complicated subsequent data processing and expensive equipment, etc.

Method used

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  • Method for preparing electrochemical luminescence sensor made of nano-composites
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  • Method for preparing electrochemical luminescence sensor made of nano-composites

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Experimental program
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Effect test

Embodiment 1

[0028] experimental reagent

[0029] Luminol (>98%) was purchased from Shanghai Biological Biotechnology Co., Ltd. Chlorauric acid (HAuCl 4 ·3H2O) was purchased from Sann Chemical Technology (Shanghai) Co., Ltd. (Annaiji). Absolute ethanol (CH 3 CH 2 OH, AR), hydrazine hydrate (N 2 h 4 ·H 2 O) and glutaraldehyde (25%, GA) were purchased from Shanghai Chemical Reagent Co., Ltd. Bovine serum albumin (BSA, purity>99.8%, molecular weight 68000) was purchased from Xiamen Xinglongda Chemical Reagent Co., Ltd. Ethyl-(3-dimethylpropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) were purchased from Sigma-Aldrich. Carcinoembryonic antigen (CEA) and CEA antibody were purchased from Shanghai Lingchao Biological Co., Ltd. The water used in the experiment was deionized water (18.2 MΩ).

[0030] 2. Preparation of AuBSA Nanospheres

[0031] Add 20 mL of chloroauric acid solution (28.43 mM) to 50 mL of 5.0 mg / mL BSA solution, and stir vigorously at room temperature for 10 min....

Embodiment 2

[0044] A method for preparing an electrochemiluminescence sensor of a nanocomposite material, using the following steps:

[0045] (1) Under stirring at room temperature, the HAuCl 4 Solution and bovine serum albumin solution are mixed evenly, and under the condition of vigorous stirring, quickly add hydrazine hydrate, HAuCl 4 1. The weight ratio between bovine serum albumin and hydrazine hydrate is 20:10:5. After the reaction is complete, age for 12 hours. After the obtained product is centrifuged, it is washed alternately with distilled water and ethanol, and finally the washed product AuBSA nanomaterial prepared by freeze-drying;

[0046] (2) Using the crosslinking effect of glutaraldehyde, the luminescent substance luminol is chemically crosslinked to the surface of the AuBSA nanomaterial, and the carboxyl group on the surface of AuBSA is further acidified to connect the CEA antibody to prepare a composite material luminol-AuBSA-anti-CEA, Specifically take the following s...

Embodiment 3

[0055] A method for preparing an electrochemiluminescence sensor of a nanocomposite material, using the following steps:

[0056] (1) Under stirring at room temperature, the HAuCl 4 Solution and bovine serum albumin solution are mixed evenly, and under the condition of vigorous stirring, quickly add hydrazine hydrate, HAuCl 4 1. The weight ratio between bovine serum albumin and hydrazine hydrate is 25:15:7. After the reaction is complete, age for 12 hours. After centrifuging the obtained product, wash it alternately with distilled water and ethanol, and finally wash the product AuBSA nanomaterial prepared by freeze-drying;

[0057] (2) Using the cross-linking effect of glutaraldehyde, the luminescent substance luminol is chemically cross-linked to the surface of the AuBSA nanomaterial, and the carboxyl group on the surface of AuBSA is further activated to connect the CEA antibody to prepare the composite material luminol-AuBSA-anti-CEA, Specifically take the following steps:...

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Abstract

The invention relates to a method for preparing an electrochemical luminescence sensor made of nano-composites. The method includes the three steps that Au-and-BSA nano-materials are prepared; luminol is crosslinked to the surfaces of the Au-and-BSA nano-materials through glutaraldehyde, carboxyl on the surfaces of the Au-and-BSA nano-materials is activated to be connected with CEA antibodies to obtain luminol-Au-and-BSA-anti-CEAs; a gold nano-layer is electrodeposited on the surface of a naked glassy carbon electrode serving as a working electrode with an electro-deposition method, the obtained luminol-Au-and-BSA-anti-CEAs are connected to the surface of the electrode, and the electrochemical luminescence immunosensor with the targeting effect on CEAs is obtained. The method is rapid, easy and convenient, and the constructed immunosensor has the advantages of being good in biocompatibility, high in specificity and sensitivity, wide in detection range and the like.

Description

technical field [0001] The invention relates to an electrochemiluminescence sensor, in particular to a preparation method of an electrochemiluminescence sensor of a nanocomposite material. Background technique [0002] Cancer is a major disease that threatens human health. According to the survey of relevant departments, malignant tumors rank second among the causes of death in my country. At present, there is still a lack of effective treatment methods to overcome cancer in the world. If effective early diagnosis of cancer can be achieved, it will be of great significance to later treatment. Early diagnosis is the main way to reduce the mortality rate of malignant tumors. Tumor markers (TumorMarker) are chemical substances that reflect the existence of tumors. Their content in tumor tissues is much higher than that in normal tissues. Their existence or quantitative changes can indicate the nature of tumors, so as to understand the histogenesis, cell Differentiation, cell ...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 贾能勤章阿敏黄楚森
Owner SHANGHAI NORMAL UNIVERSITY
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