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A detection method for simultaneous determination of oxc and metabolites mhd and mhd-g in human plasma

A metabolite and detection method technology, applied in the field of biological detection, can solve the problems of complicated processing methods and long aliasing time, and achieve the effects of high sensitivity, fast detection and strong specificity

Inactive Publication Date: 2017-01-11
WUXI PEOPLES HOSPITAL
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  • Description
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  • Application Information

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Problems solved by technology

[0005] At present, the HPLC-MS / MS method verified by the system can only detect OXC and MHD at the same time, but it also has defects: such as a long time for aliasing (10min) (High-performance liquid chromatography-tandem massspectrometry method for simultaneous quantification ofcarbamazepine, oxcarbazepine, and their main metabolites in human serum. Ther Drug Monit.2012, 34 (1): 53-58), the processing method is complex (extraction method) (Simultaneous quantitative analysis of

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  • A detection method for simultaneous determination of oxc and metabolites mhd and mhd-g in human plasma
  • A detection method for simultaneous determination of oxc and metabolites mhd and mhd-g in human plasma
  • A detection method for simultaneous determination of oxc and metabolites mhd and mhd-g in human plasma

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Experimental program
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Effect test

Embodiment

[0030] Example: Determination of Human Plasma OXC, MHD and MHD-G Concentrations

[0031] Experimental Materials and Instruments

[0032] Oxcarbazepine, purity >99.8%, batch number: 100657-201102, purchased from China National Institutes for Food and Drug Control; 10,11-dihydro-10-hydroxycarbamazepine, purity >98%, batch number: D449135, purchased from Bailingwei Company; 10,11-dihydro-10-hydroxycarbamazepine glucuronide, purity>95.5%, lot number: MD114541501, purchased from Carbosynth Company, UK; nitroazepam, lot number: 9201, purchased from National Narcotics Experiment Methanol, batch number: HX080973, acetonitrile, batch number: HX086245, all purchased from CNW Company; formic acid, batch number: T20090811, purchased from Sinopharm Chemical Reagent Co., Ltd.; water was made by ultrapure water (Milli-Q water machine).

[0033] Liquid chromatography system: ACCELA autosampler, ACCELA 1250pump, American Thermo Fisher Company; MS / MS system: TSQ QuantumAccess triple quadrupole...

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Abstract

The invention relates to a detection method for simultaneously measuring OXC in human plasma and metabolite MHD and MHD-G, and belongs to the technical field of biological detection. An experiment is carried out by taking nitrazepam as an internal standard, and can simultaneously detect the plasma concentrations of the three compounds by adopting high performance liquid chromatography-tandem mass spectrometry after pretreatment through precipitation protein. The detection method is short in time, high in specificity and sensitivity, and convenient in operation, and is successfully applied to the analysis and research of oxcarbazepine in the human plasma and the metabolite thereof.

Description

technical field [0001] The invention relates to a detection method for simultaneously measuring OXC and metabolites MHD and MHD-G in human plasma, belonging to the technical field of biological detection. Background technique [0002] Epilepsy (Epilepsy) is a common nervous system disease worldwide. Its clinical feature is brain dysfunction caused by repeated abnormal discharge of brain nerve cells. The most common and most serious disease. If it is not well controlled, it will cause irreversible damage to the cranial nerves, seriously affect the growth and development of children, and bring heavy psychological burden and economic pressure to children, families and society. [0003] Oxcarbazepine (OXC) is a new drug for the treatment of partial seizures and generalized tonic-clonic seizures. After oxcarbazepine (OXC) enters the body, hepatic cytosolic enzymes (AKR1C1, AKR1C2, AKR1C3, AKR1C4, CBR1 and CBR3) can rapidly convert it into the active metabolite 10,11-dihydro,10-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 黄凯蔡建美过湘云华颖贺晴
Owner WUXI PEOPLES HOSPITAL
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