Tissue culture and rapid propagation method for asparagus filicinus

A technology of Asparagus fern and tissue culture, applied in the field of plant reproduction, can solve the problems of low seed germination rate and low reproduction coefficient, and achieve the effect of easy rooting and clustered buds

Inactive Publication Date: 2015-12-16
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Artificial cultivation of Asparagus fern is mainly propagated by seeds and ramets, but the seed germination rate i

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] An example of the rapid propagation method of Asparagus fern tissue culture of the present invention, comprises the following steps:

[0018] (1) Selection and disinfection of explants: Take the newly germinated shoots of healthy plants of Asparagus fern as explants, remove the leaves, soak them in 2% aqueous solution of detergent for 5 minutes, and rinse them with linear tap water for 15 minutes. -30min, add 2-3 drops of Tween-20 to 100ml of 0.1% mercuric liter for disinfection for 8-10min, rinse with sterile water for 3-5 times, and finally remove surface moisture with sterile filter paper to obtain explants, in which sterile Water is distilled water through autoclaving;

[0019] (2) The first generation of explants is induced to obtain sterile test-tube plantlets: the explants obtained in step (1) are placed in the ultra-clean workbench, and cut into 1.0-1.5cm long stem segments with an axillary bud with a scalpel Inoculate into MS medium, culture for 30 days under ...

Embodiment 2

[0024] Another example of the tissue culture rapid propagation method of Asparagus fern of the present invention comprises the following steps:

[0025] (1) Selection and disinfection of explants: Take the newly germinated shoots of healthy plants of Asparagus fern as explants, remove the leaves, soak them in 2% aqueous solution of detergent for 5 minutes, and rinse them with linear tap water for 15 minutes. -30min, add 2-3 drops of Tween-20 to 100ml of 0.1% mercuric liter for disinfection for 8-10min, rinse with sterile water for 3-5 times, and finally remove surface moisture with sterile filter paper to obtain explants, in which sterile Water is distilled water through autoclaving;

[0026] (2) The first generation of explants is induced to obtain sterile test-tube plantlets: the explants obtained in step (1) are placed in the ultra-clean workbench, and cut into 1.0-1.5cm long stem segments with an axillary bud with a scalpel Inoculate into MS medium, culture for 30 days un...

Embodiment 3

[0031] Another example of the tissue culture rapid propagation method of Asparagus fern of the present invention comprises the following steps:

[0032] (1) Selection and disinfection of explants: Take the newly germinated shoots of healthy plants of Asparagus fern as explants, remove the leaves, soak them in 2% aqueous solution of detergent for 5 minutes, and rinse them with linear tap water for 15 minutes. -30min, add 2-3 drops of Tween-20 to 100ml of 0.1% mercuric liter for disinfection for 8-10min, rinse with sterile water for 3-5 times, and finally remove surface moisture with sterile filter paper to obtain explants, in which sterile Water is distilled water through autoclaving;

[0033](2) The first generation of explants is induced to obtain sterile test-tube plantlets: the explants obtained in step (1) are placed in the ultra-clean workbench, and cut into 1.0-1.5cm long stem segments with an axillary bud with a scalpel Inoculate into MS medium, culture for 30 days und...

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PUM

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Abstract

The invention provides a tissue culture and rapid propagation method for asparagus filicinus. The tissue culture and rapid propagation method comprises the following steps: (1) taking newly-germinated tender buds of asparagus filicinus strong plants as explants and disinfecting; (2) putting the disinfected explants into an MS culture medium to be induced to obtain sterile test-tube plantlets; (3) putting the sterile test-tube plantlets into an MS propagation culture medium to rapidly propagate and culture the sterile test-tube plantlets to obtain cluster buds; (4) carrying out seedling toughening on the cluster buds in an MS strong seedling culture medium to obtain strong plants; (5) putting the strong plants in an MS rooting culture medium to carry out rooting culture to obtain complete seedlings with roots; and (6) carrying out seedling hardening on the complete seedlings with the roots at a room temperature and taking out the seedlings after surface cutin is formed, transplanting the seedlings into a base material and transplanting the seedlings into a large field after the seedlings grow for one month. With the adoption of the tissue culture and rapid propagation method, high-quality asparagus filicinus germchits with the rooting rate being 80-95% can be obtained, and the large-scale seedling culture problem of asparagus filicinus is effectively solved.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a rapid propagation method of Asparagus tissue culture. Background technique [0002] Asparagus filicinus Buch.-Ham.exD.Don, also known as Artemisia crescenta, Qianhammer, Guangmaidong, Tubai, Jiuzhonggen, etc., is a perennial herb of Liliaceae. It has the effects of relieving cough in the lung, killing insects and relieving itching, and stopping hemoptysis, and has a strong antitumor effect. Asparagus fern is mostly in the wild at present, but due to over-harvesting and utilization, its resources are on the verge of depletion. [0003] The artificial cultivation of Asparagus fern is mainly propagated by seeds and ramets, but the seed germination rate is low. During ramet propagation, each plant can only get 3-5 branches with roots, and the reproductive coefficient is low. Contents of the invention [0004] The object of the present invention is to provide a method for rapid prop...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王晓峰李刚李林轩梁莹缪剑华
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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